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Regulation of Lipoprotein Metabolism in HepG2 Cells by Citrus Flavonoids

  • Elzbieta M. Kurowska
  • John A. Manthey
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 505)

Abstract

Elevated levels of blood cholesterol are known to be one of the major risk factors associated with coronary heart disease (CHD), the leading cause of death in North America. The association is largely due to the importance of cholesterol, especially low-density lipoprotein (LDL) cholesterol, in the formation and development of atherosclerotic plaque, the underlying pathological condition of CHD. Dietary intervention has been proven to play an important role in prevention and treatment of hypercholesterolemia. Common dietary strategies aimed to lower high blood cholesterol include reduced intake of dietary saturated fat and cholesterol and increased intake of fiber (Connor and Connor, 1998). Recently, many reports have proposed another approach: increased intake of certain food components and food products with cholesterol-lowering potential (Cook and Samman, 1996).

Keywords

HepG2 Cell Cholesteryl Ester Orange Juice Grapefruit Juice Concentration Of25 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ameer, B., Weintraub, R. A., Johnson, J. V., Yost, R. A., and Rouseff, R. L., 1996, Flavanone absorption after naringenin, hesperidin, and citrus administration, Clin. Pharmacol. Ther. 60: 34–40.CrossRefGoogle Scholar
  2. Anthony, M. S., Clarkson, T. B., Hughes, C. L. Jr., Morgan, T. M., and Burke, G. L., 1996, Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal Rhesus monkeys, J. Nutr. 126: 43–50.Google Scholar
  3. Bok, S.-H., Lee, S.-H., Park, Y.-B., Bae, K.-H., Son, K.-H., Jeong, T.-S., and Choi, M.-S., 1999, Plasma and hepatic cholesterol and hepatic activities of 3-hydroxyl-3-methyl-glutaryl-CoA reductase and acyl CoA:cholesterol transferase are lower in rats fed citrus peel extract or a mixture of citrus bioflavonoids, J. Nutr.. 129: 1182–1185.Google Scholar
  4. Borradaile, N. M., Carroll, K. K., and Kurowska, E. M., 1999, Regulation of HepG2 cell apolipoprotein B metabolism by the citrus flavanones hesperetin and naringenin, Lipids 34: 591–598.CrossRefGoogle Scholar
  5. Bors, W., Heller, W., Michel, C., and Saran, M., 1990, Flavonoids as antioxidants: determination of radical scavenging efficiencies, Method. Enzymol. 186: 343–355.CrossRefGoogle Scholar
  6. Choi, J. S., Yokozawa, T., and Oura, H., 1991, Antihyperlipidemic effect of flavonoids from Prunus davidiana, J. Nat. Prod. 54: 218–224.CrossRefGoogle Scholar
  7. Connor, S. L., and Connor, W. E., 1998, Pathogenic and protective nutritional factors in coronary heart disease. In: Current Prospectives on Nutrition and Health, Carroll, K. K., ed.., McGill-Queen’s University Press, Montreal, PQ, pp. 59–100.Google Scholar
  8. Cook, N.C., and Samman, S., 1996, Flavonoids — chemistry, metabolism, cardioprotective effects, and dietary sources, J Nutr. Biochem. 7: 66–76.CrossRefGoogle Scholar
  9. Guthrie, N., and Carroll, K. K., 1998a, Inhibition of mammary cancer by citrus flavonoids, In: Flavonoids in the Living System, Manthey, J. A., and Buslig, B. S., eds., Plenum Press, New York, pp. 227–236.CrossRefGoogle Scholar
  10. Guthrie, N., and Carroll, K. K., 1998b, Inhibition of human breast cancer cell growth and metastasis in nude mice by citrus juices and their constituent flavonoids, In: Biological Oxidants: Molecular Mechanisms and Health Effects, Packer, L., and Ong, A. S. H., eds., AOCS Press, Champaign, IL, pp. 257–264.Google Scholar
  11. Hansen, M. B., Nielsen, S. E., and Berg, K., 1989, Re-examination and further development of precise and rapid dye method for measuring cell growth/cell kill, J Immunol. Meth. 119: 203–210.CrossRefGoogle Scholar
  12. Kurowska, E. M., Hrabek-Smith, J. M., and Carroll, K. K., 1989, Compositional changes in serum lipoproteins during developing hypercholesterolemia induced in rabbits by cholesterol-free semipurified diets, Atherosclerosis 78:159–165.Google Scholar
  13. Kurowska, E. M., Moffatt, M., and Carroll, K. K., 1994, Dietary soybean isoflavones counteract the elevation of VLDL but not LDL cholesterol produced in rabbits by feeding a cholesterol-free, casein diet, Proc. Can. Fed. Biol. Soc. 37: 126 (Abstr.).Google Scholar
  14. Kurowska, E. M., Borradaile, N. M., Spence, J. D., and Carroll, K. K., 2000a, Hypocholesterolemic effects of dietary citrus juices in rabbits, Nutr. Res. 20 :121–129.Google Scholar
  15. Kurowska, E. M., Spence, J. D., Jordan, J., Wetmore, S., Freeman, D. J., Piche, L. A., and Senatore, P., 2000b, HDL cholesterol-raising effect of orange juice in subjects with hypercholesterolemia, Am. J. Clin. Nutr. 72: 1095–1100.Google Scholar
  16. Manthey, J. A., Grohmann, K., Montanari, A., Ash, K., and Manthey, C. L., 1999, Polymethoxylated flavones derived from citrus suppress tumor necrosis factor-a expression by human monocytes, J. Nat. Prod. 62: 441–444.CrossRefGoogle Scholar
  17. Monforte, M. T., Trovato, A., Kirjavainen, S., Forestieri, A. M., and Galati, E. M., 1995, Biological effects of hesperidin, a citrus flavonoid. (note 11): Hypolipidemic activity on experimental hypercholesterolemia in rat, Farmaco 50:595–599.Google Scholar
  18. Rajendran, S., Deepalakshimi, P. D., Parasakthy, K., Devaraj, H., and Devaraj, S. N., 1996, Effect of tincture of Crataegus on the LDL-receptor activity of hepatic plasma membrane of rats fed an atherogenic diet, Atherosclerosis 123:235–241.Google Scholar
  19. Spencer, J. P. E., Chowrimootoo, G., Choudhury, R., Debnam, E. S., Srai, S. K., and Rice- Evans, C., 1999, The small intestine can both absorb and glucuronidate luminal flavonoids, FEBS Letters 458: 224–230.CrossRefGoogle Scholar
  20. Thrift, R. N., Forte, T. M., Cahoon, B. E., and Shore, V. G., 1986, Characterization of lipoprotein produced by the human liver cell line HepG2, under defined conditions, J. Lipid Res. 27: 236–250.Google Scholar
  21. Wilcox, L. J., Borradaile, N. M., Kurowska, E. M., Telford, D. E., and Huff, M. W., 1998, Naringenin, a citrus flavonoid, markedly decreases apoB secretion in HepG2 cells and inhibits acyl CoA: cholesterol acyltransferase, Circulation 98:1–537(Abstr.).Google Scholar
  22. Yotsumoto, H., Yanagita, T., Yamamoto, K., Ogawa, Y., Cha, J. Y., and Mori, Y., 1997, Inhibitory effects of Orengedoku-to and its components on cholesteryl ester synthesis in cultured human hepatocyte HepG2 cells: evidence from the cultured HepG2 cells and in vitro assay of ACAT, Planta Med. 63:141–145.Google Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Elzbieta M. Kurowska
    • 1
  • John A. Manthey
    • 2
  1. 1.KGK Synergize, Inc.LondonCanada
  2. 2.Agricultural Research Service, Citrus and Subtropical Products LaboratoryU. S. Department of AgricultureWinter HavenUSA

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