Abstract
Thermal analysis is, in principle, a non-specific technique which thus is suitable as a general monitor for complex processes, such as cell metabolism (1). The combination of thermal analysis and highly specific enzymes offers a very powerful analytical technique. Using immobilized trypsin placed in a flow-cell in a LKBsorption microcalorimeter the enzyme-catalyzed hydrolysis of the ester substrate, BAEE, was measured (2). The calorimeter gave accurate results but the long time required for each measurement stressed the need for a more rapid analytical method for routine work. Therefore a simple, inexpensive device named “the enzyme thermistor” was constructed.
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© 1978 Springer Science+Business Media New York
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Mattiasson, B., Danielsson, B., Mosbach, K. (1978). Enzyme Thermistor Assays of Cholesterol, Oxalic Acid, Glucose and Lactose in Standard Solutions and Biological Samples. In: Pye, E.K., Weetall, H.H. (eds) Enzyme Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5163-5_51
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DOI: https://doi.org/10.1007/978-1-4757-5163-5_51
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