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Enzyme Thermistor Assays of Cholesterol, Oxalic Acid, Glucose and Lactose in Standard Solutions and Biological Samples

  • B. Mattiasson
  • B. Danielsson
  • K. Mosbach

Abstract

Thermal analysis is, in principle, a non-specific technique which thus is suitable as a general monitor for complex processes, such as cell metabolism (1). The combination of thermal analysis and highly specific enzymes offers a very powerful analytical technique. Using immobilized trypsin placed in a flow-cell in a LKBsorption microcalorimeter the enzyme-catalyzed hydrolysis of the ester substrate, BAEE, was measured (2). The calorimeter gave accurate results but the long time required for each measurement stressed the need for a more rapid analytical method for routine work. Therefore a simple, inexpensive device named “the enzyme thermistor” was constructed.

Keywords

Oxalic Acid Immobilize Enzyme Glucose Oxidase Cholesterol Oxidase General Monitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • B. Mattiasson
    • 1
  • B. Danielsson
    • 1
  • K. Mosbach
    • 1
  1. 1.Chemical Center, Biochemical Division University of LundSweden

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