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Immobilization of Enzymes by Reductive Alkylation

  • G. P. Royer
  • F. A. Liberatore

Abstract

Reductive alkylation in which Schiff’s bases formed between an aldehyde and an amino group may be reduced with sodium borohydride (NaBH4) or sodium cyanoborohydride is a convenient and gentle method to specifically introduce alkyl groups into proteins at ε-amino groups of lysine (1–4). We have used this reaction to couple enzymes to carbohydrate supports which had been oxidized with sodium metaperiodate (NaI04) including dextran-coated, controlled-pore glass (Dextran-CPG), Glycophase-CPG and Sepharose. These are neutral, hydrophilic supports with a highly porous structure. Glycophase-CPG is porous glass coated with a glyceryl silane (Glass≡Si(CH2)3OCH2CHOHCH2OH).

Keywords

Amino Acid Analysis Porous Glass Sodium Cyanoborohydride Sodium Metaperiodate Trypsin Immobilization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • G. P. Royer
    • 1
  • F. A. Liberatore
    • 1
  1. 1.Department of BiochemistryThe Ohio State UniversityColumbusUSA

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