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Prospects for an Assay Technique Based on Volatile Enzyme Products

  • J. C. Weaver

Abstract

After considering a simple mathematical model (1) similar to that used for the thermal enzyme probe (2), it was decided to explore (3) an assay technique in which immobilized enzyme catalyzed reactions yielding volatile products are interfaced to a relatively inexpensive mass spectrometer, as shown in Fig. 1.

Keywords

Immobilize Enzyme Volatile Product Simple Mathematical Model Assay Technique Unstirred Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    WEAVER, J.C. Quarterly Progress Report No. 114, Research Laboratory of Electronics, MIT, July 15, 1974, p. 8.Google Scholar
  2. 2.
    COONEY, C.L., WEAVER, J.C., TANNENBAUM, S.R., FALLER, D.V., SHIELDS, A., and Jahnke, M. In “Enzyme Engineering, Vol 2” (Ed. E.K. Pye and L.B. Wingard, Jr.) Plenum Press, New York, 1974, p. 411.CrossRefGoogle Scholar
  3. 3.
    WEAVER, J.C., MASON, M.K., JARRELL, J.A. & PETERSON, J.W. Progress Report No. 115, Research Laboratory of Electronics, MIT, January, 1975, p.9; and Biochim. Biophys. Acta 438: 296, 1976.CrossRefGoogle Scholar
  4. 4.
    ZABORSKY, O.R. “Immobilized Enzymes” Chemical Rubber Co. Press, Cleveland, Ohio, 1973.Google Scholar
  5. 5.
    WEAVER, J.C. & KING, J.G. Proc. Nati. Acad. Sci. U.S., 70: 2781, 1973Google Scholar
  6. 6.
    BEAN, C.P. & WARD, W.J. Private Communications.Google Scholar
  7. 7.
    JARRELL, J.A., WEAVER, J.C. & KING, J.G. Progress Report No. 115, Research Laboratory of Electronics, MIT, January, 1975, p. 4.Google Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • J. C. Weaver
    • 1
  1. 1.Research Laboratory of Electronics and Physics DepartmentMassachusetts Institute of TechnologyCambridgeUSA

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