The Cholesterol Electrode: Use of the Polarographic Oxidase Anode with Multiple Enzymes

  • L. C. ClarkJr.
  • C. Emory
  • C. J. Glueck
  • M. Campbell


In the past two decades the relationship between cholesterol, triglyceride and atherosclerosis has been established and clarified (1). Initial ascertainment of an elevation of plasma cholesterol or triglyceride leads to a more detailed evaluation of lipid and lipoprotein classes useful in diagnosis and treatment (1). Screening procedures for quantitation of plasma cholesterol are highly important as a primary approach to an amelioration of atherosclerosis. Because of the close association of cholesterol and atherosclerosis (1), many approaches to the measurement of plasma cholesterol have been examined (2–4). Currently, colorimetric methods involving an isopropanol extraction and saponification are widely used and have been automated to allow reasonable precision and accuracy at a rate of 30 samples/h (4). The calorimetric methodology is expensive, relatively non-specific, subject to interference from even slight changes in humidity and bilirubin levels, and requires the use of highly corrosive reagents such as concentrated sulfuric acid, acetic anhydride and acetic acid (2,4,5). Colorimetric methods also require at least 5–10 cc blood sampling with the requisite venipuncture.


Plasma Cholesterol Cholesterol Ester Cholesterol Oxidase Enzyme Electrode Electrode Response 
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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • L. C. ClarkJr.
    • 1
  • C. Emory
    • 1
  • C. J. Glueck
    • 1
  • M. Campbell
    • 1
  1. 1.Children’s Hospital Research FoundationCincinnatiUSA

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