Skip to main content

Strategy for Pure Hydrophobic Chromatography

  • Chapter

Abstract

Hydrophobic adsorption chromatography as a generally useful methodology for the purification of enzymes, is emerging as a logical complement to the well established ion-exchange chromatography. It is even tempting to suggest that in time the use of hydrophobic adsorption methods could eclipse the presently popular affinity methods for enzyme purification.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. KAUZMANN, W. Adv. Prot. Chem. 24: 1, 1959.

    Article  Google Scholar 

  2. TANFORD, C. “The Hydrophobic Effect”, J. Wiley, New York, 1973.

    Google Scholar 

  3. KLOTZ, I.M. Fed. Proc. 24: S24, 1965.

    Google Scholar 

  4. MUKERJEE, P. Advan. Colloid Interface Sci. 1: 241, 1967.

    Article  Google Scholar 

  5. FISCHER, H.F. Proc. Natl. Acad. Sci. USA 51: 1285, 1964.

    Article  Google Scholar 

  6. BIGELOW, C.C. J. Theoret. Biot. 26: 187, 1967.

    Article  Google Scholar 

  7. ANFINSEN, C.B. & SCHERAGA, H.A. Adv. Prot. Chem. 29: 205, 1975.

    Article  Google Scholar 

  8. KLOTZ, I.M. Arch. Biochem. Biophys. 238: 704, 1970.

    Article  Google Scholar 

  9. LEE, B. & RICHARDS, F.M. J. Mol. Biol. 55: 379, 1971.

    Article  Google Scholar 

  10. HOFSTEE, B.H.J. Biochem. Biophys. Res. Commun. 63: 618, 1975.

    Article  Google Scholar 

  11. TANFORD, C. Adv. Prot. Chem. 23: 122, 1968.

    Google Scholar 

  12. FRANKS, F. In “Water, a Comprehensive Treatise” (Ed. F. Franks) Vol. 4, Plenum Press, New York, 1975 p.l.

    Google Scholar 

  13. SARGENT, R. & RIEMAN, W. J. Phys. Chem. 62: 354, 1957.

    Article  Google Scholar 

  14. DETERMANN, H. & LAMPERT, K. J. Chromatogr. 69: 123, 1972.

    Article  Google Scholar 

  15. NISHIKAWA, A.H. & BAILON, P. Anal. Biochem. 64: 268, 1975.

    Article  Google Scholar 

  16. ER-EL, Z., ZAIDENZAIG, Y. & SHALTIEL,S. Biochem. Biophys. Les. Commun. 49: 383, 1972.

    Google Scholar 

  17. RIMERMAN, R.A. & HATFIELD, G.W. Science 182: 1268, 1973.

    Article  Google Scholar 

  18. DOELLGAST, G.J. & FISHMAN, W.H. Biochem. J. 142: 103, 1974.

    Google Scholar 

  19. RAIBAND, O., HOGBERG-RAIBAND, A. & GOLDBERG, M. E. FEBS Lett. 50: 130, 1975.

    Google Scholar 

  20. BIGELIS, R. & UMBARGER, H. E. J. Biol. Chem. 250: 4315, 1975.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1978 Springer Science+Business Media New York

About this chapter

Cite this chapter

Nishikawa, A.H. (1978). Strategy for Pure Hydrophobic Chromatography. In: Pye, E.K., Weetall, H.H. (eds) Enzyme Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5163-5_40

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-5163-5_40

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-5165-9

  • Online ISBN: 978-1-4757-5163-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics