Purification of Proteinases by Affinity Chromatography Techniques
During studies on the microbial production of acid or alkaline proteinases and an investigation of the similarities of these proteinases from the view point of enzyme-inhibitor relationships, we were successful in isolating pepsin and alkaline proteinase inhibitors. The pepsin inhibitor we isolated was found to be an acylpentapeptide which was named SPI (pepsin-inhibitor produced by Streptomyces)(1). Alkaline proteinase inhibitor, on the other hand, was found to be a protein and was abbreviated as SSI (subtilisin BPN′-inhibitor produced by Streptomyces)(2). We tried to immobilize these inhibitors and to purify the proteinases by affinity chromatography on both immobilized inhibitors.
KeywordsAlkaline Proteinase Acid Proteinase Amino Radical Acid Proteinase Activity Crystalline Enzyme
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
MURAO, S. & SATOI, S. Agr. Biol. Chem
. 34:1265, 1970, and ibid.
35: 1477, 1971.Google Scholar
MURAO, S., SATO, S. & MUTO, N. Agr. Biol. Chem.
36: 160, 1972.CrossRefGoogle Scholar
FUKUMURA, M., SATOI, S., KUWANA, N. & MURAO, S. Agr. Biol. Chem.
35: 1310, 1971.CrossRefGoogle Scholar
SATO, S. & MURAO, S. Agr. Biol. Chem
. 37:1067, 1973, and ibid.
38: 587, 1974.Google Scholar
AXÉN, R., PORATH, J. & ERNBACK, S. Nature
, 2l4: 1302, 1967.Google Scholar
CUATRECASAS, P., WILCHEK, M. & ANFINSEN, C.B. Proc. Nat’l. Acad. Sci.
, 61: 636, 1968.CrossRefGoogle Scholar
SATO, S., KIMURA, T. & MURAO, S. Agr. Biol. Chem.
, 39: 415, 1975.CrossRefGoogle Scholar
INOUYE, K., TONOMURA, B. & HIROMI, K. Agr. Biol. Chem.
39: 1159, 1975.CrossRefGoogle Scholar
© Springer Science+Business Media New York 1978