Unit Operations of Enzyme Purification

  • C. W. Hancher


The successful purification of an enzyme from a bacterial or tissue source usually follows a general separation flowsheet consisting of the following general processing steps: (1) Starting material preparation and cell harvesting, which includes tissue removal, grinding, cellular disruption and centrifugation to produce a crude extract; (2) Crude extract separation, which includes DEAE, or other charged support, either fractional or total product precipitation, or product separation by size, gel exclusion, or membrane sizing; (3) Secondary separation of the partially purified product, which usually is a combination of the above separation steps; (4) Pre- and post-treatment of the product (e.g. salt removal, water removal, and pH adjustment) to prepare for the next separation operation, which is frequently time-consuming and results in a possibility of product loss.


Hydraulic Head DEAE Cellulose Water Removal DEAE Column Salt Removal 


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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • C. W. Hancher
    • 1
  1. 1.Chemical Technology DivisionOak Ridge National LaboratoryOak RidgeUSA

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