Coupled and Multienzyme-Nylon Tube Reactors

  • P. V. Sundaram


One of the recent developments in enzyme technology was the demonstration that a nylon tube with enzymes bound to its inside surface may be used as a catalytic reactor coil for analytical purposes by using the flow through principle (1). Since then, this principle has been extended to many enzyme systems. A natural consequence of this early work has been the study of sequential reactions involving more than one enzyme. Here two coupled enzyme systems, namely Pyruvate Kinase — Lactate Dehydrogenase (PK — LDH) and Alkaline Phosphatase — Adenosine Deaminase (AP — ADA) are described indicating how more enzymes may be added to these systems to act in conjunction and to form multienzyme systems.


Pyruvate Kinase Adenosine Deaminase Radioactive Compound Pyruvate Kinase Activity Routine Estimation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Sundaram, P.V. and Hornby, W. E. FEBS Lett. 10: 325, 1970.CrossRefGoogle Scholar
  2. 2.
    Hornby, W.E., Campbell, J., INMAN, D.J. and MORRIS, D.L. In “Enzyme Engineering, Vol 2” (Ed. E.K. Pye & L.B.Wingard Jr.) Plenum Press, 1974,p. 401.Google Scholar
  3. 3.
    Sundaram,P.V. Nucleic Acid Res. 1: 1587, 1974.CrossRefGoogle Scholar
  4. 4.
    Sundaram, P.V. Biochim. Biophys. Acta (in the press).Google Scholar
  5. 5.
    Sundaram, P.V. Nucleic Acid Res. (in the press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • P. V. Sundaram
    • 1
  1. 1.Max-Planck-Institut für Experimentelle MedizinGöttingenGermany

Personalised recommendations