Endotoxin pp 445-466 | Cite as

Identification and Characterization of Lipopolysaccharide Receptor Molecules on Mammalian Lymphoid Cells

  • M.-G. Lei
  • L. Flebbe
  • D. Roeder
  • D. C. Morrison
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)


Since the discovery by Neter in the late 1950’s that bacterial endotoxic lipopolysaccharides (LPS) will bind readily to cells of mammalian origin (reviewed in 15), the concept of specific receptors for LPS has intrigued endotoxin researchers. A variety of immunologic, biochemical and morphologic approaches have been adopted in order to demonstrate experimentally the existence of such LPS receptors; in general however, such efforts have been confounded by relatively high levels of nonspecific binding to mammmalian cell membrane components (reviewed in 16). A specific membrane localized high affinity LPS binding lipoglycoprotein present on human erythrocytes was identified by Springer and his colleagues in 1970 (24, 25), although efforts to extend these observations to human platelets and leukocytes or murine lymphocytes were less successful (26, 27). More recent efforts by several investigators have provided strong evidence for receptors on distinct cell subpopulations for carbohydrate components of specific preparations of LPS, (reviewed in 7), but the specific receptor for the structurally conserved, biologically active lipid A component of LPS has, to date, not been defined.


Peripheral Blood Mononuclear Cell Lymphoid Cell Coomassie Blue Inbred Mouse Strain Splenic Macrophage 


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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • M.-G. Lei
    • 1
  • L. Flebbe
    • 1
  • D. Roeder
    • 1
  • D. C. Morrison
    • 1
  1. 1.Department of MicrobiologyUniversity of Kansas Medical CenterKansas CityUSA

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