Mechanisms of Neutralization of Endotoxin by Monoclonal Antibodies to O and R Determinants of Lipopolysaccharide
The protective potentials of antibodies to O and R core regions of lipopolysaccharide (LPS) have been amply substantiated (2, 7). However, the efficacy of antibodies to the toxic lipid A moiety itself is still ambiguous, and how antibodies to regions distal from lipid A can neutralize the toxicity remains to be clarified. We have compared the effects of mouse monoclonal antibodies (mAbs) of IgG class to these three regions of LPS on the biological activities as well as micellic structure of LPS, in order to shed light on the mechanism of neutralization of endotoxin by these antibodies.
KeywordsPGE2 Production Sucrose Density Gradient Protective Potential Endotoxin Shock Toxic Lipid
Unable to display preview. Download preview PDF.
- 1.Aggarwall, B. B., Kohr, W. J., and Harkins, R. N., 1985, Human tumor necrosis factor. Production, purification and characterization. J. Biol. Chem. 260: 2345.Google Scholar
- 2.Braude, A. I., Douglas, H., and Ziegler, E. J., 1977, Antibody to cell wall glycolipid of gram-negative bacteria: Induction of immunityto bacteremia and endotoxemia. J. Infect. Dis. 5136: 5167.Google Scholar
- 3.Galanos, C., Luderitz, O., and Westphal, O., 1971, Preparation and properties of antisera against the lipid A component of bacterial lipopolysaccharides. Eur. J. Biochem. 24: 110.Google Scholar
- 5.Oi, V. T., and Herzenberg, L. A., 1908, Immunoglobulin-producing hybrid cell lines. in: “Selected methods in cellular immunology”. B. B. Michell, ed., W. H. Freeman Co., San Francisco.Google Scholar