Endotoxin pp 301-317 | Cite as

Interaction of Bacterial Endotoxin (LPS) with Fluid Phase and Macrophage Membrane Associated C1q, the FC-Recognizing Component of the Complement System

  • M. Loos
  • B. Euteneuer
  • F. Clas
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)


The bactericidal activity of normal serum was first described by Buchner in 1889 (10). This effect is abolished when serum has been incubated for 30 min at 56°C. Gram positives are less sensitive than Gram negative bacteria to direct killing, although gram positive cocci are opsonized by the action of serum mediated by antibodies and complement (22). It was found that most of the smooth strains of gram negative bacteria are serum resistant; whereas, the corresponding rough forms are extremely serum sensitive (32, 37). Thus evidence was provided that the composition of the bacterial surface may influence the reaction of the bacteria with the lytic system. The bacteriolytic properties of serum are mediated by the so called MAC (Membrane Attack Complex). This hydrophobic complex is inserted into cell membranes as a diner and produces lysis (24). The antibody-dependent activation of the classical complement pathway as well as the activation of the alternative complement pathway by bacteria has been extensively studied. However, several bacterial strains are rapidly killed in non-immune sera. Furthermore, it was reported that Cl is absorbed to Mycoplasma pneumoniae in the absence of antibodies. The direct interaction with Cl and the activation of the classical complement cascade had even more biological consequences for these bacteria than activation of the alternative pathway (8).


Complement System Lysosomal Enzyme Negative Bacterium Chronic Granulomatous Disease Classical Pathway 


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  1. 1.
    Aaskov, J. G. and Halliday, W. J., 1971, Requirement for lymphocyte-macrophage interaction in the response of mouse spleen cultures to pneumococcae polysaccharide. Cell Immunol. 2: 335.PubMedCrossRefGoogle Scholar
  2. 2.
    Aderem, A. A., Cohen, D. S., Wright, S. D. and Cohn, Z. A., 1986, Bacterial lipopolysaccharides prime macrophages for enhanced release of arachidonic acid metabolites. J. Exp. Med. 164: 165.Google Scholar
  3. 3.
    Allison, A. C. and Davis, P., 1975, Increased biochemical and biological activities of mononuclear phagocytes exposed to various stimuli, with special reference to secretion of lysosomal enzymes, in: “Mononuclear phagocytes in immunity, infection and pathology”, R. van Furth, ed., Blackwell Scientific Publications, Oxford, p. 487.Google Scholar
  4. 4.
    Ando, M., Suga, M., Sugimoto, M. and Tokuomi, H., 1979, Superoxide production in pulmonary alveolar macrophages and killing of BCG by the super-oxide-generating system with or without catalase. Infect. Immunol. 24: 404.Google Scholar
  5. 5.
    Babior, B. M., 1978, Oxygen-dependent microbial killing by phagocytes. N. Engl. J. Med. 298: 659–668.Google Scholar
  6. 6.
    Berkel, I. A., Loos, M., Sanal, O., Mauff, G., Gungen, Y., Ors, V., Ersoy, F. and Yegin, O., 1979, Clinical and immunological studies in a case of selective complement Clq deficiency. Clin. Exp. Immunol. 38: 52–63.Google Scholar
  7. 7.
    Bjornson, A. B. and Bjornson, H. W., 1977, Activation of complement by opportunist pathogens and chemotypes of Salmonella minnesota. Infect. Immun. 16: 748–753.Google Scholar
  8. 8.
    Bredt, W., Wellek, B., Brunner, H. and Loos, M., 1977, Studies on the interaction between myoplasma pneumoniae and the first component of complement. Infect. Immun. 15: 7–12.Google Scholar
  9. 9.
    Buchner, H., 1889, Uber die bakterientötende Wirkung des zellfreien Blutserums. Zentralbi. Bakteriol. 5: 817–823.Google Scholar
  10. 10.
    Burger, R., 1988, Complement biosynthesis: Factors of the alterna-tive pathway, in: “The Complement System,” K. Rother and G. O. Till, eds., Springer Verlag, Heidelberg, p. 70–80.Google Scholar
  11. 11.
    Clas, F. and Loos, M., 1981, Antibody-independent binding of the first component of complement (C1) and its subcomponent Clq to the S- and R-forms of Salmonella minnesota. Infect. Immun. 31: 1138–1144.Google Scholar
  12. 12.
    Cole, F. S. and Colten, H. R., 1988, Complement biosynthesis: Factors of the classical pathway, in: “The Complement System,” K. Rother and G. O. Till, eds., Springer Verlag, Heidelberg, p. 44–70.Google Scholar
  13. 13.
    Euteneuer, B., Störkel, S. and Loos, M., 1986, Contributions of Clq, bacterial lipopolysaccharide and porins during attachment and ingestion phases of phagocytosis by murine macrophages. Infect. Immun. 51: 807–815.Google Scholar
  14. 14.
    Fishman, W. H., Kato, K., Antiss, C. L. and Green, S., 1967, Human serum ß-glucuronidase; its measurement and some of its properties. Clin. Chim. Acta. 15: 435.Google Scholar
  15. 15.
    Friedberg, D. and Shilo, M., 1970, Role of cell wall structure of Salmonella in the interaction with phagocytes. Infect. Immun. 2: 279.Google Scholar
  16. 16.
    Galanos, C., Luderitz, 0., Rietschel, E. T. and Westphal, 0., 1977, Newer aspects of the chemistry and the biology of bacterial lipopolysaccharides with special reference to their lipid A component, in: “Biochemistry of lipids II”, T. W. Goodwin, ed., University Park Press, Baltimore, 14: 239–335.Google Scholar
  17. 17.
    Gery, I., Gershon, R. K. and Waksman, B. H., 1972, Potentiation of the T-lymphocyte response to mitogens. 1. The responding cell. J. Exp. Med. 136: 128.Google Scholar
  18. 18.
    Gewurz, H., Shin, H. S. and Mergenhagen, S. E., 1968, Interactions of the complement sytem with endotoxic lipopolysaccharide: consumption of each of six terminal complement components. J. Exp. Med. 128: 1049–1057.Google Scholar
  19. 19.
    Heinz, H.-P., Dlugonska, H., Rüde, E. and Loos, M., 1984, Monoclonal anti-mouse macrophage antibodies recognize the globular portions of Clq, a subcomponent of the first component of complement. J. Immunol. 133: 400–404.PubMedGoogle Scholar
  20. 20.
    Hirsch, J. G. and Cohn, Z. A., 1960, Degranulation of polymorphonuclear phagocytes following phagocytosis of microorganisms. J. Exp. Med. 112: 1005–1014.Google Scholar
  21. 21.
    Johnston, R. B., 1978, Oxygen metabolism and the microbicidal activity of macrophages. Fed. Prod. 37: 2759–2764.Google Scholar
  22. 22.
    Johnston, R. B., Klemperer, M., Alper, C. A. and Rosen, R. S., 1969, The enhancement of bacterial phagocytosis by serum. The role of complement components and two cofactors. J. Exp. Med. 129: 1275–1290.Google Scholar
  23. 23.
    Johnston, R. B., Godzik, C. A. and Cohn, Z. A., 1978, Increased superoxide anion production by immunologically activated and chemically elicited macrophages. J. Exp. Med. 148: 115.Google Scholar
  24. 24.
    Kolb, W. B. and Müller-Eberhard, H. J., 1975, The membrane attack mechanism of complement isolation and subunit composition of the C5b-9 complex. J. Exp. Med. 141: 724–735.Google Scholar
  25. 25.
    Loos, M., 1982, Antibody-independent activation of Cl, the first component of complement. Ann. Immunol. ( Inst. Pasteur ) 133C: 165–179.Google Scholar
  26. 26.
    Loos, M., 1983, Biosynthesis of the collagen-like Clq molecule and its receptor functions for Fc and polyanionic molecules on macrophages. Curr. Top. Microbiol. Immunol. 102: 1–56.Google Scholar
  27. 27.
    Loos, M., Muller, W., Boltz-Nitulescu, G. and Förster, 0., 1980, Evidence that Clq, a subcomponent of the first component of complement is an Fc-receptor of peritoneal and alveolar macrophages. Immunobiol. 157: 54.CrossRefGoogle Scholar
  28. 28.
    Loos, M., Wellek, B., Thesen, R. and Opferkuch, W., 1978, Antibody-independent interaction of the first component of complement with gram-negative bacteria. Infect. Immun. 22: 5–9.Google Scholar
  29. 29.
    Lowrie, D. B., Aber, V. R. and Carrol, M. E. W., 1979, Division and death rates of Salmonella typhimurium inside macrophages. Use of penicillin as a probe. J. Gen. Microbiol. 110: 409.Google Scholar
  30. 30.
    Martin, H.. Heinz, H.-P., Reske, K. and Loos, M.. 1987. Macrophage Clq: Characterization of a membrane form of Clq and of multimers of Clq subunits. J. Immunol. 138: 3863.Google Scholar
  31. 31.
    Müller, W., Hanauske-Abel. H. and Loos, M., 1978. Biosynthesis of the first component of complement by human and guinea pig peritoneal macrophages. Evidence for an independent production of the Cl subunits. J. Immunol. 121: 1578.Google Scholar
  32. 32.
    Muschel, L.-H. and Larsen, L. L., 1970. The sensitivity of smooth and rough gram-negative bacteria to the immune bactericidal reaction. Proc. Soc. Exp. Biol. Med. 133: 345–348.Google Scholar
  33. 33.
    Nathan, C. F. and Root, R. K., 1977, Hydrogen peroxide release from mouse peritoneal macrophages: Dependence on sequential activation and triggering. J. Exp. Med. 146: 1648.Google Scholar
  34. 34.
    Pantalone, R. and Page, R. L., 1977, Enzyme production and secretion by lymphokine activated macrophages. J. Reticuloendothel. Soc. 21: 343.PubMedGoogle Scholar
  35. 35.
    Rapp, H. J. and Borsos, T., 1970, Molecular basis of complement action. Appleton-Century-Crofts, New York.Google Scholar
  36. 36.
    Root, R. K., Ellman, L. and Frank, M. M.. 1972, Bactericidal and opsonic properties of C4-deficient guinea pig serum. J. Immunol. 109: 477–486.PubMedGoogle Scholar
  37. 37.
    Rowley, D.. 1968, Sensitivity of rough gram-negative bacteria to the bactericidal action of serum. J. Bacteriol. 95: 1647–1650.Google Scholar
  38. 38.
    Schnyder, J. and Baggiolini. M., 1978, Secretion of lysosomal hydrolases by stimulated and non-stimulated macrophages. J. Exp. Med. 148: 435.Google Scholar
  39. 39.
    Schnyder, J. and Baggiolini, M., 1978, Role of phagocytosis in the activation of macrophages. J. Exp. Med. 148: 1449.Google Scholar
  40. 40.
    Smith, K. A., Lachman. L. B. and Oppenheim, J. J., 1980, The functional relationship of the interleukins. J. Exp. Med. 151: 1551.Google Scholar
  41. 41.
    Stahelin, H.. Suter, E. and Karnovsky, M. L., 1956. Studies on the interaction between phagocytes and tubercle bacilli I. Observations on the metabolism of guinea pig leukocytes and the influence of phagocytosis. J. Exp. Med. 104: 121.Google Scholar
  42. 42.
    Stossel. T. P., 1975. Phagocytosis: recognition and ingestion. Semin. Hematol. 12: 83–116.Google Scholar
  43. 43.
    Yagawa, K., Kaku, M., Ichinose, Y., Nagao, S., Tanaka, A., Aida, Y. and Tomoda, A., 1985, Down-regulation of Fc-receptor expression in guinea pig peritoneal exudate macrophages by muramyl dipeptide or lipopolysaccharide. J. Immunol. 134: 3705.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • M. Loos
    • 1
  • B. Euteneuer
    • 1
  • F. Clas
    • 1
  1. 1.Institute of Medical MicrobiologyJohannes Gutenberg-UniversityMainzGermany

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