A New Endotoxin-Specific Assay
After its development by Levin and Bang in 1968 (4), the limulus amebocyte lysate test had been considered specific for endotoxin until Kakinuma et al., found that carboxymethylated (1→3) −β -D-glucan also activates limulus coagulation enzymes (3). Morita et al., showed that amebocyte lysate contains, in addition to endotoxin-sensitive factor C, a factor G that is sensitive for a trace amount of (1→3)− β -D-glucan (Fig 1a) (5).
KeywordsSerratia Marcescens Horseshoe Crab Systemic Fungal Infection Shigella Flexneri Limulus Amebocyte Lysate
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- 1.Iwanaga, S., Morita, T., Harada, T., et al., 1978, Chromogenic substrat- es for horseshoe crab clotting enzyme. Its application for the assay of bacterial endotoxins. Haemostasis 7: 183–188.Google Scholar
- 2.Iwanaga, S., Morita, T., Miyata, T.. et al., 1984, The limulus coagulation system sensitive to bacterial endotoxins. in: “Bacterial endotoxin: chemical, biological, and clinical aspects.” J. Y. Homma et al., eds. Weinheim: Verlag Chemie GmbH, 365–382.Google Scholar
- 3.Kakinuma, A., Asano, T., Torii, H., and Sugino, Y., 1981. Gelation of Limulus amebocyte lysate by an antitumor (1-3) -13 -D-glucan. Biochem. Biophys. Res. Commun. 101: 434–439.Google Scholar
- 5.Morita, T., Tanaka, S., Nakamura, T., and Iwanaga, S., 1981, A new (1β3)-ß -D-glucan-mediated coagulation pathway found in Limulus amebocytes. FEBS Lett 129: 318–321.Google Scholar