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Endotoxin pp 141-145 | Cite as

Cloning and Analysis of rfb Gene Synthesizing the Mannan O Side Chain of Escherichia coli 09 Lipopolysaccharide

  • N. Kido
  • M. Ohta
  • H. Ito
  • K-I. Iida
  • Y. Arakawa
  • T. Komatsu
  • K. Jann
  • N. Kato
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)

Summary

The rfb gene encoding the proteins responsible for the synthesis of the repeating units (0 side-chain) of Escherichia coli 09 lipopolysaccharide was cloned into a conjugative plasmid RP4: :miniMu and was expressed in E. coli K-12.

Keywords

Adjuvant Activity Shigella Dysenteriae Synthetic Liquid Medium Shiga Toxin Gene Typical Ladder Pattern 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • N. Kido
    • 1
  • M. Ohta
    • 1
  • H. Ito
    • 1
  • K-I. Iida
    • 1
  • Y. Arakawa
    • 1
  • T. Komatsu
    • 1
  • K. Jann
    • 1
    • 2
  • N. Kato
    • 1
  1. 1.Department of BacteriologyNagoya University School of MedicineAichi 466Japan
  2. 2.Max-Planck-Institut fur ImmunbiologieFreiburgGermany

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