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Selective DNA Repair in the Transcribed Strands of Active Genes in Mammalian Cells

  • Isabel Mellon
  • Graciela Spivak
  • Philip C. Hanawalt
Chapter

Abstract

Cyclobutane pyrimidine dimers are preferentially removed from transcriptionally active genes: (i) In Chinese hamster cells which remove only a small fraction of dimers from their total DNA, dimers are efficiently removed from the transcriptionally active dihydrofolate reductase (DHFR) gene and poorly removed from a sequence near the gene (Bohr et al., 1985). (ii) In mouse cells which also remove only a small fraction of dimers from their total DNA, dimers are proficiently removed from the transcriptionally active c-abl gene and poorly removed from the inactive c-mos gene (Madhani et al., 1986). (iii) In repair proficient human cells, dimers are removed more rapidly from the active DHFR gene than from bulk DNA or from the nontranscribed repetitive alpha sequences (Mellon et al., 1986). This work was recently reviewed by Smith (1987).

Keywords

Upstream Gene Pyrimidine Dimer Cyclobutane Pyrimidine Dimer DHFR Gene Enzyme Treated Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Isabel Mellon
    • 1
  • Graciela Spivak
    • 1
  • Philip C. Hanawalt
    • 1
  1. 1.Department of Biological SciencesStanford UniversityStanfordUSA

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