A Shuttle Vector for Investigating the Molecular Mechanisms of Mutagenesis in Mammalian Cells in Culture

  • E. Dogliotti
  • K. Ellison
  • F. Palombo
  • J. Essigmann


The understanding of the mechanisms by which mutations are fixed as a heritable change in mammalian cells is at present quite limited. In an attempt to dissect the process, recombinant DNA techniques have been recently successfully applied (1–6). Recombinant genomes, that replicate both in mammalian cells and bacteria, are referred to as shuttle vectors. Analysis of the mutational pattern of marker genes, or of genetic elements, resident upon shuttle vector genomes has began to provide information on the processing of DNA damage in mammalian cells.


Chinese Hamster Ovary Cell Shuttle Vector Xeroderma Pigmentosum Chinese Hamster Ovary Cell Line Recombinant Genome 


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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • E. Dogliotti
    • 1
    • 2
  • K. Ellison
    • 2
  • F. Palombo
    • 1
  • J. Essigmann
    • 2
  1. 1.Laboratory of Applied ToxicologyIstituto Superiore di Sanita’RomaItaly
  2. 2.Department of Applied Biological SciencesMassachusetts Institute of TechnologyCambridgeUSA

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