Molecular Mechanisms of Mutagenesis Induced by Chemical Carcinogens and CIS-DDP
The conversion of DNA lesions into mutations is an active biochemical process. Due to the remarquable efficiency of the error free repair mechanisms only a very small number of DNA lesions will eventually be processed into a mutation. Under normal conditions, less than one percent of DNA lesions give rise to mutations. This makes the biochemical study of the mechanisms involved in mutagenesis very difficult. As a first molecular approach, the study of the mutationnal specificity of a given mutagen will provide important informations concerning the mechanisms that are involved. This is particularly true if the analysis of the mutational specificity can be performed in hosts having altered genotypes for repair and (or) mutagenesis. Bacteria are in this respect the organisms of choice, due to the large number of existing repair, replication and recombination mutants.
KeywordsFrameshift Mutation Tetracycline Resistance Gene Single Base Pair Substitution Base Substitution Mutation Premutagenic Lesion
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- 8.D. BURNOUF, M.P. DAUNE and R.P.P. FUCHS, Proc. Natl. Acad. Sci 84 3758–62(1987)Google Scholar
- 10.R. P. P. FUCHS, J.-F. LEFEVRE, J. POUYET and M. P. DAUNE, Biochemistry 15,, 3347–3351 (1976).Google Scholar
- 12.E.SAGE and M. LENG, Nucl. Acids Res. 2, 1241–1250 (1981)Google Scholar
- 15.R.D.WELLS, J.J. MIGHETTA, J. KLYSIK, J.E. LARSON, S.M. STIRDIVANT and W.ZACHARIAS, J. Biol. Chem. 257, 10166–10171 (1982).Google Scholar
- 16.M. P. DAUNE, R.P.P. FUCHS and M. LENG, Natl. Cancer Inst. Monogr. 58, 201–210 (1981).Google Scholar
- 18.N. KOFFEL-SCHWARTZ, G. MAENHAUT-MICHEL and R.P.P. FUCHS, J. Mol. Biol. 193, 651–659 (1987)Google Scholar
- 21.R. M. MYERS, L. S. L.RMAN and T. MANIATIS,Science 229, 242–247 (1985)Google Scholar