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Regulation of MAPK pathways in response to purinergic stimulation of adult rat cardiac myocytes

  • Chapter
Cardiac Cell Biology

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 39))

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Abstract

We investigated the activation of mitogen-activated protein kinases (MAPKs) pathways by purinergic stimulation in cardiac myocytes from adult rat hearts. ATPγS increased the phosphorylation (activation) of the extracellular signal regulated kinase 1 and 2 (ERK1/2) and p38 MAPK. ERK1/2 and p38 MAPK activation was differential, ERK1/2 being rapid and transient while that of p38 MAPK slow and sustained. Using selective inhibitors, activation of ERK1/2 was shown to involve protein kinase C and MEK1/2 while that of p38 MAPK was regulated by both protein kinase C and protein kinase A. Further more, we show that purinergic stimulation induces the phosphorylation of the MAPK downstream target; mitogen- and stress-activated protein kinase 1 (MSK1), in cardiac myocytes. The time course of MSK1 phosphorylation closely follows that of ERK activation. Inhibitors of the ERK and p38 MAPK pathways were tested on the phosphorylation of MSK1 at two different time points. The results suggest that ERKs initiate the response but both ERKs and p38 MAPK are required for the maintenance of the complete phosphorylation of MSK1. The temporal relationship of MSK1 phosphorylation and cPLA2 translocation induced by purinergic stimulation, taken together with previous findings, is an indication that cPLA2 may be a downstream target of MSK1. (Mol Cell Biochem 242: 163–171, 2003)

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Abbreviations

ERK:

extracellular signal-regulated kinase

MAPK:

mitogen-activated protein kinase

MSK1:

mitogen- and stress-activated protein kinase-1

PKA:

protein kinase A

PKC:

protein kinase C

PKI:

cAMP dependent protein kinase inhibitor

PLA2 :

phospholipase A2

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Author information

Authors and Affiliations

  1. Laboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Thomais Markou & Antigone Lazou

  2. INSERM U-390, Physiopathologie Cardiovasculaire, CHU Arnaud de Villeneuve, Montpellier, France

    Guy Vassort

  3. Laboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, 54006, Greece

    Antigone Lazou

Authors
  1. Thomais Markou
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  2. Guy Vassort
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  3. Antigone Lazou
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Editor information

Editors and Affiliations

  1. Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Elissavet Kardami  & Larry Hryshko  & 

  2. Institute of Cardiovascular Sciences and Division of Stroke and Vascular Disease, St. Boniface General Hospital Research Centre and Department of Physiology, University of Manitoba, Winnipeg, Canada

    Nasrin Mesaeli

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Markou, T., Vassort, G., Lazou, A. (2003). Regulation of MAPK pathways in response to purinergic stimulation of adult rat cardiac myocytes. In: Kardami, E., Hryshko, L., Mesaeli, N. (eds) Cardiac Cell Biology. Developments in Molecular and Cellular Biochemistry, vol 39. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-4712-6_21

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  • DOI: https://doi.org/10.1007/978-1-4757-4712-6_21

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4419-5324-7

  • Online ISBN: 978-1-4757-4712-6

  • eBook Packages: Springer Book Archive

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