Abstract
The extent of cardiac myosin light chain phosphorylation was measured during regional myocardial ischemia in the dog. A multiple-projectile cutter was used to sample adjacent biopsies from the normal and ischemic areas of the myocardium in an open-chested dog heart following 30 min of coronary artery ligation. Measurement of metabolite levels and blood flow in the individual biopsies clearly defined the border zone between normal and ischemic myocardium. Myosin light chain phosphorylation was measured after isoelectric focusing of biopsy samples and subsequent densitometric analysis. A 50% increase in phosphorylation was observed in the ischemic zone which may correlate with the reduced contractility which is a feature of the ischemic myocardium.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adelstein, R. S. 1980. Phosphorylation of muscle contractile proteins. Fed. Proc.39:1544–1546.
Barany, K., Barany, M., Gillis, J. M., and Kushmerick, M. J. 1980. Myosin light chain phosphorylation during the contraction cycle of frog muscle. Fed. Proc.39:1547–1551.
Cummins, P., Price, K. M., and Littler, W. A. 1980. Foetal myosin light chain in human ventricle. J. Muscle Res. Cell Motility1:357–366.
Fishbein, M. C, Hare, C. A., Gissen, S. A., Spadoro, J., Maclean, D., and Maroko, P. R. 1980. Identification and quantification of histochemical border zones during the evolution of myocardial infarction in the rat. Cardiovasc. Res.14:41–49.
Frearson, N., and Perry, S. V. 1975. Phosphorylation of the light-chain components from cardiac and red skeletal muscles. Biochem. J.151:99–107.
Hearse, D. J. 1980. Oxygen deprivation and early myocardial contractile failure. A reassessment of the possible role of adenosine triphosphate. Am. J. Cardiol.44:1115–1119.
Hearse, D. J., Opie, L., Katzeff, I. E., Lubbe, W. F., Van der Werff, T. J., Peisach, M., and Boulle, G. 1977. Characterization of the ‘border zone’ in acute regional ischemia in the dog. Am. J. Cardiol.40:716–726.
Hearse, D. J., Yellon, D. M., Chappell, D. A., Wyse, R. K. H., and Ball, G. A. 1981. A high velocity impact device for obtaining multiple, contiguous myocardial biopsies. J. Mol. Cell. Cardiol.13:197–206.
Holroyde, M. J., Small, D. A. P., Howe, E., and Solaro, R. J. 1979. Isolation of cardiac myofibrils and myosin light chains with in vivo levels of light chain phosphorylation. Biochim. Biophys. Acta587:628–637.
Jeacocke, S. A., and England, P. J. 1980. Phosphorylation of myosin light chains in perfused rat heart. Biochem, J. 188:763–768.
Kopp, S. J., and Barany, M. 1979. Phosphorylation of the 19,000-dalton light chain of myosin in perfused rat heart under the influence of negative and positive inotropic agents. J. Biol. Chem.254:12007–12012.
Kubier, W., and Katz, A. M. 1977. Mechanism of early pump failure of the ischaemic heart: Possible role of adenosine triphosphate depletion and energie phosphate accumulation. Am J. Cardiol.40:467–471.
Lubbe, W. F., Peisach, M., Pretorius, R., Brugreel, K. J. J., and Opie, L. H. 1974. Distribution of myocardial blood flow before and after coronary artery ligation in the baboon. Relation to early ventricular fibrillation. Cardiovasc. Res.8:478–487.
O’Farrell, P. H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem.250:4007–4021.
Perrie, W. T., Smillie, L. B., and Perry, S. V. 1973. A phosphorylated light-chain component of myosin from skeletal muscle. Biochem. J.135:151–164.
Puri, P. S. 1974. Modification of experimental myocardial infarct size by cardiac drugs. Am. J. Cardiol.33:521–528.
Small, J. V., and Sobieszek, A. 1977. Ca-regulation of mammalian smooth muscle acto-myosin via a kinase-phosphatase dependent phosphorylation and dephosphorylation of the 20,000 M light chain of myosin. Eur. J. Biochem.76:521–530.
Stull, J. T., Manning, D. R., High, C. W., and Blumenthal, D. K. 1980. Phosphorylation of contractile proteins in heart and skeletal muscle. Fed. Proc.39:1552–1557.
Theroux, P., Franklin, D., Ross, J., Jr., and Kemper, W. S. 1974. Regional myocardial function during acute coronary artery occlusion and its modification by pharmacologic agents in the dog. Circ. Res.35:896–908.
Vatner, S. F. 1980. Correlation between acute reductions in myocardial blood flow and function in conscious dog. Circ. Res.47:201–207.
Yellon, D. M. 1979. A multiple biopsy gun for the study of three-dimensional metabolic geometry. J. Physiol.293:5–6.
Yellon, D. M., Hearse, D. J., Crewe, R., Grannell, J., and Wyse, R. K. H. 1980. Characterization of the interface between normal and ischemic tissue during acute myocardial infarction. Am. J. Cardiol.47:1233–1239.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1983 Springer Science+Business Media New York
About this chapter
Cite this chapter
Cummins, P., Yellon, D.M., Hearse, D.J. (1983). Myosin Light Chain Phosphorylation during Regional Myocardial Ischemia. In: Chazov, E., Saks, V., Rona, G. (eds) Advances in Myocardiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-4441-5_45
Download citation
DOI: https://doi.org/10.1007/978-1-4757-4441-5_45
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-4443-9
Online ISBN: 978-1-4757-4441-5
eBook Packages: Springer Book Archive