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Detection of Intracellular Anoxia and Its Relationship to Onset of Hemodynamic Dysfunction and ST-Segment Elevation in the Intact Dog Heart

  • D. W. Baron
  • C. E. Harrison

Abstract

The temporal relationships among onset of cellular anoxia after coronary artery occlusion, contractile dysfunction, and electrocardiographic ischemia were studied in dogs with an intact circulation. Nicotinamide adenine dinucleotide (NADH) fluorescence was used to detect intracellular anoxia, using a fiber-optic method. Paired NADH concentrations from ischemic (394 ± 10 |xmol/g) and normoxic (285 ± 11 |xmol/g) regions of the heart were obtained, and the differences (A[NADH]) were correlated with compensated fluorescence (r = 0.76, P a 0.01). Onset of fluorescence occurred 1 to 2 sec after coronary artery occlusion, followed by hemodynamic (5 sec) and electrocardiographic (13 sec) changes. These data indicate that intracellular anoxia, with alterations in redox potential, is not synchronous with the onset of contractile failure and provide indirect support for intracellular acidosis as the likely mediator of contractile failure.

Keywords

Myocardial Blood Flow Coronary Artery Occlusion Contractile Dysfunction Pyridine Nucleotide Coronary Artery Ligation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • D. W. Baron
    • 1
  • C. E. Harrison
    • 1
  1. 1.Mayo Clinic and Mayo FoundationCardiac Muscle Research LaboratoryRochesterUSA

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