Release of Prostacyclin into the Coronary Venous Blood in Patients with Coronary Arterial Disease

  • L. Kaijser
  • J. Nowak
  • C. Patrono
  • Å. Wennmalm


Voluntary patients with a history of myocardial infarction and with typical effort angina underwent catheterization of the coronary sinus and a brachial artery. Healthy young males, serving as controls, were subjected to the same procedure. Arterial and coronary venous blood was drawn at rest and during atrial pacing to angina (patients) or to a heart rate of 140 beats/min (healthy volunteers) for analysis of 6-ketoprostaglandin F (6-keto-PGF) and pros-tacyclin-like activity (PILA). 6-Keto-PGF levels were measured using radioimmunoassay; PILA in the blood was assayed by rapid preparation of platelet-rich plasma followed by determination of the ADP-induced platelet aggregation. Increased arterial levels of PILA and of radioimmunoactive 6-keto-PGF (RIA-6-keto-PGF) were observed in the patients at rest as well as during pacing. No obvious release of RIA-6-keto-PGF occurred at rest, either in the patients or in the controls. However, during pacing, increased amounts of RIA-6-keto-PGF appeared in the coronary venous blood of the patients. The results demonstrate that an increased cardiac prostacyclin formation prevails in patients with signs of impaired coronary flow and suggest that ischemic heart disease is characterized by an insufficient vascular response to this vasodilator prostaglandin rather than by its insufficient endogenous production.


Platelet Aggregation Coronary Sinus Coronary Blood Flow Atrial Pace Healthy Young Male 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Afonso, S., Bandow, G. T., and Rowe, G. G. 1974. Indomethacin and the prostaglandin hypothesis of coronary blood flow regulation. J. Physiol. (Lond). 241:299–308.Google Scholar
  2. 2.
    Alexander, R. W., Kent, K. M., Pisano, J. J., Keiser, H. R., and Cooper, T. 1975. Regulation of post-occlusive hyperemia by endogenously synthesized prostaglandins in the dog heart. J. Clin. Invest. 55:1174–1181.PubMedCrossRefGoogle Scholar
  3. 3.
    Berger, H. J., Zaret, B. L., Speroff, L., Cohen, L. S., and Wolfson, S. 1976. Regional cardiac prostaglandin release during myocardial ischemia in anesthetized dogs. Circ. Res. 38:566–571.PubMedCrossRefGoogle Scholar
  4. 4.
    Berger, H. J., Zaret, B. L., Speroff, L., Cohen, L. S., and Wolfson, S. 1977. Cardiac prostaglandin release during myocardial ischemia induced by atrial pacing in patients with coronary artery disease. Am. J. Cardiol. 30:481–486.CrossRefGoogle Scholar
  5. 5.
    Block, A. J., Feinberg, H., Herbaczynska-Cedro, K., and Vane, J. R. 1975. Anoxia-induced release of prostaglandins in rabbit isolated hearts. Circ. Res. 36:34–42.PubMedCrossRefGoogle Scholar
  6. 6.
    Born, G. V. R., and Cross, M. J. 1963. Inhibition of the aggregation of blood platelets by substances related to adenosine diphosphate. J. Physiol. (Lond.) 166:29P-30P.Google Scholar
  7. 7.
    De Deckere, E. A. M., Nugteren, D. H., and Ten Hoor, F. 1977. Prostacyclin is the major prostaglandin released from the isolated perfused rabbit and rat heart. Nature 268:160–163.PubMedCrossRefGoogle Scholar
  8. 8.
    Fox, A. C, Reed, G. E., Glassman, E., Kaltman, A. J., and Silk, B. B. 1974. Release of adenosine from human hearts during angina induced by rapid atrial pacing. J. Clin. Invest. 53:1447–1457.PubMedCrossRefGoogle Scholar
  9. 9.
    Giles, R. W., and Wilcken, D. E. 1977. Reactive hyperaemia in the dog heart: Inter-relations between adenosine, ATP, and aminophylline and the effect of indomethacin. Cardiovasc. Res. 11:113–121.PubMedCrossRefGoogle Scholar
  10. 10.
    Gryglewski, R. J., Bunting, S., Moncada, S., Flower, R. J., and Vane, J. R. 1976. Arterial walls are protected against deposition of platelet thrombi by a substance (prostaglandin X) which they make from prostaglandin endoperoxides. Prostaglandins 12:685–715.PubMedGoogle Scholar
  11. 11.
    Gryglewski, R., Dembinska-Kiec, A., Zmuda, A., and Gryglewska, T. 1978. Prostacyclin and thromboxane A2 biosynthesis capacities of heart, arteries and platelets at various stages of experimental artherosclerosis. Atherosclerosis 31:385–394.PubMedCrossRefGoogle Scholar
  12. 12.
    Gryglewski, R., Korbut, R., and Ocetkiewicz, A. 1978. Generation of prostacyclin by lungs in vivo and its release into the arterial circulation. Nature 273:765–766.PubMedCrossRefGoogle Scholar
  13. 13.
    Gryglewski, R., Korbut, R., Ocetkiewicz, A., Splawinski, J., Wojtaszek, B., and Swies, J. 1978. Lungs as a generator of prostacyclin—hypothesis on physiological significance. Naunyn Schmiedebergs Arch. Pharmacol. 304:45–50.PubMedCrossRefGoogle Scholar
  14. 14.
    Hintze, T. H., and Kaley, G. 1977. Prostaglandins and the control of blood flow in the canine myocardium. Circ. Res. 40:313–320.PubMedCrossRefGoogle Scholar
  15. 15.
    Jenkins, C. S. P., Caen, J. P., Vainer, H., and Pokutecky, J. 1972. Inhibition of adenosine uptake by platelets. Nature [New Biol.] 23:210–211.Google Scholar
  16. 16.
    Johnson, R. A., Morton, D. R., Kinner, J. H., Gorman, R. R., McGuire, J. C, and Sun, F. F. 1976. The chemical structure of prostaglandin X (prostacyclin). Prostaglandins 12:915–928.PubMedGoogle Scholar
  17. 17.
    Jørgensen, M. A., Stoffersen, E., and Dyerberg, J. 1979. Stability of prostacyclin in plasma. Lancet 1:1352.PubMedCrossRefGoogle Scholar
  18. 18.
    Kalsner, S. 1977. The effect of hypoxia on prostaglandin output and tone in isolated coronary arteries. Can. J. Physiol. Pharmacol. 55:882–887.PubMedCrossRefGoogle Scholar
  19. 19.
    Kent, K. M., Alexander, R. W., Pisano, J. J., Keiser, H. R., and Cooper, T. 1973. Prostaglandin dependent coronary vasodilator responses. Physiologist 16:361.Google Scholar
  20. 20.
    Kraemer, R. J., and Folts, J. D. 1973. Release of prostaglandin following temporary occlusion of the coronary artery. Fed. Proc. 32:454.Google Scholar
  21. 21.
    Kraemer, R. J., Phernetton, T. M., and Folts, J. D., 1976. Prostaglandin-like substances in coronary venous blood following myocardial ischemia. J. Pharmacol. Exp. Ther. 199:611–619.PubMedGoogle Scholar
  22. 22.
    Metha, J., Metha, P., and Pepine, C. J. 1978. Platelet aggregation in aortic and coronary venous blood in patients with and without coronary disease. 3. Role of tachycardia stress and propranolol. Circulation 58:881–886.CrossRefGoogle Scholar
  23. 23.
    Moncada, S., Korbut, R., Bunting, S., and Vane, J. R. 1978. Prostacyclin is a circulating hormone. Nature 273:767–768.PubMedCrossRefGoogle Scholar
  24. 24.
    Morcillio, E., Reid, P. R., Dubin, N., Ghodgaonkar, R., and Pitt, B. 1980. Myocardial prostaglandin E release by nitroglycerin and modification by indomethacin. Am. J. Cardiol. 45:53–57.PubMedCrossRefGoogle Scholar
  25. 25.
    Owen, T. L., Ehrhart, I. C, Weidner, W. J., Scott, J. B., and Haddy, F. J. 1975. Effect of indomethacin on local blood flow regulation in canine heart and kidney. Proc. Soc. Exp. Biol. Med. 149:871–876.PubMedGoogle Scholar
  26. 26.
    Patrono, C, Wennmalm, A., Ciabattoni, G., Nowak, J., Pugliese, F., and Cinotti, G. A. 1979. Evidence for an extra-renal origin of urinary prostaglandin E2 in healthy men. Prostaglandins 18:623–629.PubMedGoogle Scholar
  27. 27.
    Sinzinger, H., Silberbauer, K., Wagner, O., Winter, M., and Auerswald, W. 1978. Prostacyclin—preliminary results with vascular tissue of various species and its importance for atherosclerotic involvement. Atherogenesis 3:123–136.Google Scholar
  28. 28.
    Swies, J., Radomski, M., and Gryglewski, R. J. 1979. Angiotensin-induced release of prostacyclin (PGI2) into circulation of anaesthetized cats. Pharmacol. Res. Commun. 11:649–655.PubMedCrossRefGoogle Scholar
  29. 29.
    Wennmalm, A., Pham-Huu-Chanh, and Junstad, M. 1974. Hypoxia causes prostaglandin release from perfused rabbit hearts. Acta Physiol. Scand. 91:133–135.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • L. Kaijser
    • 1
    • 2
    • 3
  • J. Nowak
    • 1
    • 2
    • 3
  • C. Patrono
    • 1
    • 2
    • 3
  • Å. Wennmalm
    • 1
    • 2
    • 3
  1. 1.Department of Clinical PhysiologyHuddinge Hospital, Karolinska HospitalStockholmSweden
  2. 2.St. Erik’s HospitalStockholmSweden
  3. 3.Department of PharmacologyCatholic UniversityRomeItaly

Personalised recommendations