Release of Prostacyclin into the Coronary Venous Blood in Patients with Coronary Arterial Disease
Voluntary patients with a history of myocardial infarction and with typical effort angina underwent catheterization of the coronary sinus and a brachial artery. Healthy young males, serving as controls, were subjected to the same procedure. Arterial and coronary venous blood was drawn at rest and during atrial pacing to angina (patients) or to a heart rate of 140 beats/min (healthy volunteers) for analysis of 6-ketoprostaglandin F1α (6-keto-PGF1α) and pros-tacyclin-like activity (PILA). 6-Keto-PGF1α levels were measured using radioimmunoassay; PILA in the blood was assayed by rapid preparation of platelet-rich plasma followed by determination of the ADP-induced platelet aggregation. Increased arterial levels of PILA and of radioimmunoactive 6-keto-PGF1α (RIA-6-keto-PGF1α) were observed in the patients at rest as well as during pacing. No obvious release of RIA-6-keto-PGF1α occurred at rest, either in the patients or in the controls. However, during pacing, increased amounts of RIA-6-keto-PGF1α appeared in the coronary venous blood of the patients. The results demonstrate that an increased cardiac prostacyclin formation prevails in patients with signs of impaired coronary flow and suggest that ischemic heart disease is characterized by an insufficient vascular response to this vasodilator prostaglandin rather than by its insufficient endogenous production.
KeywordsPlatelet Aggregation Coronary Sinus Coronary Blood Flow Atrial Pace Healthy Young Male
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