Advertisement

Effects of Propranolol and Hydrocortisone Pretreatment on Radiation-Induced Myocardial Injury in Rats

  • M. R. Tajuddin
  • S. K. Johri
  • M. Tariq
  • V. Ram

Abstract

Earlier studies in our laboratory (23) showed evidence of dose-related acute injury to the myocardium after exposure of rats to ionizing radiation. Biochemical, histological, and electrocardiographic parameters were studied. In further continuation of this study, the effects of intervention by pretreatment with propranolol (10 mg/kg body weight) and hydrocortisone (10 mg/kg body weight) have been studied. The above drugs were administered to male albino rats weighing 150 to 200 g 30 min before exposure to 6000-rad single-dose γ radiation over the precordial area. The parameters observed were cardiac glycogen, serum enzymes, lactate, pyruvate, blood sugar, adrenal ascorbic acid, and histology of the myocardium. The beneficial effects of this procedure are discussed.

Keywords

Glycogen Store Cardiac Metabolism Pyruvate Level Electrocardiographic Parameter Adrenal Glucocorticoid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Asatoor, A.M., and King, E.J. 1954. Simplified colorimetric blood sugar method. Biochem. J. 56:44.Google Scholar
  2. 2.
    Bing, R. J. 1965. Cardiac metabolism. Physiol. Rev. 45:171–225.PubMedGoogle Scholar
  3. 3.
    Burch, G. E., Sohal, R. S., Sun, S. C, Miller, G. C, and Colcolough, H. L. 1968. Effects of radiation on the human heart. Arch. Int. Medicine 21:P230.CrossRefGoogle Scholar
  4. 4.
    Calva, E., Mujica, A., Bisteni, A., and Sodi-Pallares, D. 1965. Oxidative phosphorylation in cardiac infarct. Effect of glucose-KCl-insulin solution. Am. J. Physiol. 209:371–375.PubMedGoogle Scholar
  5. 5.
    Dluhy, R. G., Lauler, D. P., and Thorn, W. T. 1973. Symposium on steroid therapy— pharmacology and chemistry of adrenal glucocorticoids. Med. Clin. North Am. 57(5): 1155–1165.PubMedGoogle Scholar
  6. 6.
    Friedeman, T. E., and Greaser, J. B. 1935. The determination of lactic acid. J. Biol. Chem. 100:291–308.Google Scholar
  7. 7.
    Groover, M. E., Jeringan, J. A., and Martin, C. D. 1960. Variation in serum lipid concentration and clinical coronary disease. Am. J. Med. Sci. 237:133–139.CrossRefGoogle Scholar
  8. 8.
    Hamarsten, J. F., Charles, W., Cathey, R., Remond, F., and Wolf, S. 1957. Serum cholesterol, diet, and stress in patients with coronary artery disease. J. Clin. Invest. 36:897.Google Scholar
  9. 9.
    Havel, R. J., Carlson, L. A., Ekelund, L. G., and Holmgren, A. 1964. Studies on the relation between mobilization of free fatty acids and energy metabolism in man—Effects of norepinephrine and nicotinic acid. Metabolism 13:1402.PubMedCrossRefGoogle Scholar
  10. 10.
    Kinney, J. M., Long, C. L., and Duke, J. H. 1970. Carbohydrate and nitrogen metabolism after injury. Ciba Found. Symp 105–126.Google Scholar
  11. 11.
    Leaf, A. 1973. Cell swelling, a factor in ischaemic tissue injury. Circulation 48:455–458.PubMedCrossRefGoogle Scholar
  12. 12.
    Montgomery, R. 1957. Determination of glycogen. Arch. Biochem. Biophys. 67:378–386.PubMedCrossRefGoogle Scholar
  13. 13.
    Opie, L. 1980. Cardiac metabolism, catecholamines, calcium, cyclic AMP, and substrates. In: M. Tadjuddin, P. K. Das, M. Tariq, and N. S. Dhalla (eds.), Advances in Myocardiology, Vol. 1, pp. 3–20. University Park Press, Baltimore.Google Scholar
  14. 14.
    Rabb, W. 1944. Cardiotoxic substances in the blood and heart muscle in uraemia. J. Lab. Clin. Med. 29:715.Google Scholar
  15. 15.
    Reitman, S., and Frankel, S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28:56–70.PubMedGoogle Scholar
  16. 16.
    Roe, J. H., and Kuether, C. A. 1943. The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid. J. Biol. Chem. 147:399–407.Google Scholar
  17. 17.
    Scheuer, J. 1967. Myocardial metabolism in cardiac hypoxia. Am. J. Cardiol. 19:385–392.PubMedCrossRefGoogle Scholar
  18. 18.
    Scheuer, J. 1972. Pathogenic significance of disproportion between myocardial oxygen supply and consumption. Myocardiology 1:721.Google Scholar
  19. 19.
    Smithen, C, Christodoulon, J., and Brachfield, N. 1974. Protective role of increased myocardial glycogen stores induced by propranolol. In: 7 th Annual Meeting of the International Study Group for Research in Cardiac Metabolism, Quebec, June 18–21. Google Scholar
  20. 20.
    Stewart, J. R., and Fajardo, L. F. 1971. Radiation induced disease: Clinical and experimental aspects. Radiol. Clin. North Am. 9:511.PubMedGoogle Scholar
  21. 21.
    Stewart, J. R., Fajardo, L. F., Cohen, K. E., and Page, V. 1968. Experimental radiation induced heart disease in rabbits. Radiology 91:814–817.Google Scholar
  22. 22.
    Stoner, H. B. 1958. Studies on the mechanism of shock. The quantitative aspects of glycogen metabolism after limb ischemia in the rat. Br. J. Exp. Pathol. 39:635–651.PubMedGoogle Scholar
  23. 23.
    Tajuddin, M. R., Kumar, S., Tariq, M., Tyagi, S. P., and Gupta, R. 1980. Effect of radiation on myocardial metabolism and structure. In: M. Tajuddin, P. K. Das, M. Tariq, and N. S. Dhalla (eds.), Advances in Myocardiology. Vol. 2, pp. 244–238. University Park Press, Baltimore.Google Scholar
  24. 24.
    Tajuddin, M., Tariq, M., Bilgrami, N. L., and Kumar, S. 1980. Biochemical and pathological changes in the heart following bile duct ligation. In: M. Tajuddin, P. K. Das, M. Tariq, and N. S. Dhalla (eds.), Advances in Myocardiology. Vol. 2, pp. 209–212. University Park Press, Baltimore.Google Scholar
  25. 25.
    Wroblesskl, F., and La Due, S. 1956. Serum glumatic pyruvic transaminase in cardiac and hepatic disease. Proc. Soc. Exp. Biol. Med. 91:569–571.Google Scholar
  26. 26.
    Zlatkis, A., Zab, B., and Boyle, J. A. 1953. A new method for the direct determination of serum cholesterol. J. Clin. Med. 41:486.Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • M. R. Tajuddin
    • 1
  • S. K. Johri
    • 1
  • M. Tariq
    • 1
  • V. Ram
    • 1
  1. 1.Faculty of MedicineAligarh Muslim UniversityAligarhIndia

Personalised recommendations