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Brain-Specific Proteins: S-100 Protein, 14-3-2 Protein, and Glial Fibrillary Protein

  • Blake W. Moore

Abstract

The differentiated forms and functions of a specialized cell are expressed through the properties of its individual proteins, properties which are determined by their primary structure. Nervous system cells, having extremely specialized functions, are among the most highly differentiated of all types of cells. Therefore, it is important to know which proteins are specific to nervous system cells since these particular proteins would be related to specific functions within the nervous system, such as propagation of action potentials, synaptic transmission involving several chemical transmitters— each with its associated processes of synthesis, inactivation, release, and receptor activity, establishment of specific pathways and connections, action of supportive cells such as various types of glia, and many other functions.

Keywords

Nervous System Cell Beef Brain Fibrous Astrocyte Brain Specific Protein Single Tryptophan Residue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ansborg, R., and Neuhoff, V., 1971, Micro-disc electrophoresis of brain proteins. III. Heterogeneity of the nervous specific proteins S-100, Int. J. Neurosci. 2: 151–160.CrossRefGoogle Scholar
  2. Benda, P., Lightbody, J., Sato, G., Levine, L., and Sweet, W., 1968, Differentiated rat glial cell strain in tissue culture, Science 161: 370–371.PubMedCrossRefGoogle Scholar
  3. Bennet, G., 1974, Immunologic and electrophoretic identity between nervous system specific proteins antigen alpha and 14–3–2, Brain Res. 68: 365–369.CrossRefGoogle Scholar
  4. Bennet, G. S., and Edelman, G., 1968, Isolation of an acidic protein from rat brain, J. Biol. Chem. 243: 6234–6241.Google Scholar
  5. Bignami, A., and Dahl, D., 1973, An immunofluorescence study with antibodies to a protein specific to astrocytes, Brain Res. 49: 393–403.PubMedCrossRefGoogle Scholar
  6. Bignami, A., Eng, L. F., Dahl, D., and Uyeda, C. T., 1972, Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence, Brain Res. 43: 429–435.PubMedCrossRefGoogle Scholar
  7. Bogoch, S., 1969, Proteins, in Handbook of Neuro chemistry, Vol. 1, pp. 75–92, Plenum Press, New York.Google Scholar
  8. Bogoch, S., 1970, Glycoproteins of the brain of the training pigeon, in Protein Metabolism of the Nervous System (A. Lajtha, ed), pp. 555–569, Plenum Press, New York.CrossRefGoogle Scholar
  9. Calissano, P., and Bangham, A. D., 1971, Effect of two brain specific proteins (S-100 and 14–3–2) on cation diffusion across artificial lipid membranes, Biochem. Biophys. Res. Commun. 43: 504–509.PubMedCrossRefGoogle Scholar
  10. Calissano, P., Moore, B. W., and Friesen, A., 1969, Effect of calcium ion on S-100, protein of the nervous system, Biochemistry 8: 4318–4326.PubMedCrossRefGoogle Scholar
  11. Cicero, T. J., and Moore, B. W., 1970, Turnover of the brain specific protein, S-100, Science 169: 1333–1334.PubMedCrossRefGoogle Scholar
  12. Cicero, T. J., and Provine, R. R., 1972, The levels of the brain specific proteins, S-100 and 14–3–2, in the developing chick spinal cord, Brain Res. 44: 294–298.PubMedCrossRefGoogle Scholar
  13. Cicero, T. J., Cowan, W. M., and Moore, B. W., 1970a, Changes in the concentrations of the two brain specific proteins, S-100 and 14–3–2, during the development of the avian optic tectum, Brain Res. 24: 1–10.PubMedCrossRefGoogle Scholar
  14. Cicero, T. J., Cowan, W. M., Moore, B. W., and Suntzeff, V., 1970b, The cellular localization of the two brain specific proteins, S-100 and 14–3–2, Brain Res. 18: 25–34.PubMedCrossRefGoogle Scholar
  15. Cicero, T. J., Ferendelli, J. A., Suntzeff, V., and Moore, B. W., 1972, Regional changes in CNS levels of the S-100 and 14–3–2 proteins during development and aging of the mouse, J. Neurochem. 19: 2119–2125.PubMedCrossRefGoogle Scholar
  16. Cotman, C, and Matthews, D. A., 1971, Synaptic plasma membranes from rat brain synapto- somes: Isolation and partial characterization, Biochem. Biophys. Acta 249: 380–394.PubMedCrossRefGoogle Scholar
  17. Dahl, D., and Bignami, A., 1973, Glial fibrillary protein from normal human brain, purification and properties, Brain Res. 57: 343–360.PubMedCrossRefGoogle Scholar
  18. Dannies, P. S., and Levine, L., 1969, Demonstration of subunits in beef brain acidic protein (S-100), Biochem. Biophys. Res. Commun. 37: 587–592.PubMedCrossRefGoogle Scholar
  19. Dannies, P. S., and Levine, L., 1971a, Structural properties of bovine brain S-100 protein, J. Biol. Chem. 246: 6276–6283.PubMedGoogle Scholar
  20. Dannies, P. S., and Levine, L., 19716, The role of sulfhydryl groups in serological properties of bovine brain S-100 protein, J. Biol. Chem. 246: 6284–6287.Google Scholar
  21. DeLores Arnai, R., Aberice, M., and deRobertis, E., 1967, Ultrastructural and enzymic studies of cholinergic and non-cholinergic synaptic membranes isolated from brain cortex, J. Neurochem. 14: 215–225.CrossRefGoogle Scholar
  22. Eichberg, J., Wittaker, V. P., and Dawson, R. M. C, 1964, Distribution of lipids in subcellular particles of guinea pig brain, Biochem. J. 92: 91–100.PubMedGoogle Scholar
  23. Eng, L. T., Vanderhaegen, J. J., Bignami, A., and Gerstl, B., 1971, An acidic protein isolated from fibrous astrocytes, Brain Res. 28: 351–354.PubMedCrossRefGoogle Scholar
  24. Gombos, G., Vincendon, G., Tardy, J., and Mandel, P., 1966, Hétérogénéité électrophorétique et préparation rapide de la fraction protéique S-100, C.R. Soc. Biol. F. D268: 1533–1535.Google Scholar
  25. Herschman, H. R., 1971, Synthesis and degradation of a brain-specific protein (S-100 protein) by clonal cultured human glial cells, J. Biol. Chem. 246: 7569–7571.PubMedGoogle Scholar
  26. Hyden, H., and McEwen, B., 1966, A glial protein specific for the nervous system, Proc. Natl. Acad. Sci. (U.S.) 55: 354–358.CrossRefGoogle Scholar
  27. Kessler, D., Levine, L., and Fasman, G., 1968, Some conformational and immunological properties of a bovine brain acidic protein (S-100), Biochemistry 1: 158–764.Google Scholar
  28. Lerner, M. P., and Herschman, H. R., 1972, S-100 protein synthesis by isolated polyribosomes from rat brain, Science 178: 995–996.PubMedCrossRefGoogle Scholar
  29. Levine, L., and Moore, B. W., 1965, Structural relatedness of a vertebrate brain acidic protein as measured immunochemically, Neurosci. Res. Prog. Bull. 3: 18–22.Google Scholar
  30. McEwen, B., and Hyden, H., 1966, A study of specific brain proteins on the semi-micro scale, J.Neurochem. 13: 823–833.PubMedCrossRefGoogle Scholar
  31. Moore, B. W., 1965, A soluble protein characteristic of the nervous system, Biochem. Biophys. Res. Commun. 19: 739–744.PubMedCrossRefGoogle Scholar
  32. Moore, B. W., 1969, Acidic proteins, in Handbook of Neurochemistry, Vol. 1, pp. 93–99, Plenum Press, New York.Google Scholar
  33. Moore, B. W., 1972, Chemistry and biology of two proteins, S-100 and 14–3–2, specific to the nervous system, in International Review of Neurobiology, Vol. 15, pp. 215–225, Academic Press, New York.CrossRefGoogle Scholar
  34. Moore, B. W., 1973, Brain specific proteins, in Proteins of the Nervous System (D. J. Schneider, ed.), pp. 1–12, Raven Press, New York.Google Scholar
  35. Moore, B. W., and McGregor, D., 1965, Chromatographic and electrophoretic fractionation of soluble proteins of brain and liver, J. Biol. Chem. 240: 1647–1653.PubMedGoogle Scholar
  36. Moore, B. W., and Perez, V. J., 1966, Complement fixation for antigens on a picogram level, J. Immunol. 96: 1000–1005.PubMedGoogle Scholar
  37. Moore, B. W., and Perez, V. J., 1968, Specific acidic proteins of the nervous system, in Physiological and Biochemical Aspects of Nervous Integration (F. D. Carlson, ed.), pp. 343–360, Prentice-Hall, Englewood Cliffs, N.J.Google Scholar
  38. Moore, B. W., Perez, V. J., and Gehring, M., 1968, Assay and regional distribution of a soluble protein characteristic of the nervous system, J. Neurochem. 15: 265–272.PubMedCrossRefGoogle Scholar
  39. Morgan, I. G., Wolfe, L. S., Mandel, P., and Gombos, G., 1971, Isolation of plasma membranes from rat brain, Biochim. Biophys. Acta 241: 737–751.PubMedCrossRefGoogle Scholar
  40. Perez, V. J., and Moore, B. W., 1968, Wallerian degeneration in rabbit tibial nerve</b>: Changes in amounts of the S-100 protein, J. Neurochem. 15: 971–977.Google Scholar
  41. Perez, V. J., Olney, J. W., Cicero, T. J., Moore, B. W., and Bahn, B. A., 1970, Wallerian degeneration in rabbit optic nerve</b>: Cellular localization in the central nervous system of the S-100 and 14–3–2 proteins, J. Neurochem. 17: 511–519.Google Scholar
  42. Pfeiffer, S. E., Kornblith, P. L., Cares, H. L., Seals, J., and Levine, L., 1972, S-100 protein in human acoustic neurinomas, Brain Res. 41: 187–193.PubMedCrossRefGoogle Scholar
  43. Schneider, D. J., 1973, Studies of nervous system proteins, in Proteins of the Nervous System, pp. 67–94, Raven Press, New York.Google Scholar
  44. Schubert, D., Heinemann, S., Carlisle, W., Tarikas, H., Kimes, B., Patrick, J., Steinback, J. H., Culp, W., and Brandt, B. L., 1974, Clonal cell lines from the rat central nervous system, Nature 249: 224–227.PubMedCrossRefGoogle Scholar
  45. Uozumi, T., and Ryan, R. J., 1973, Isolation, amino acid composition and radioimmunoassay of human brain S-100 protein, Mayo Clin. Proc. 48: 50–56.PubMedGoogle Scholar
  46. Uyeda, C. T., Eng, L. F., and Bignami, A., 1972, An immunological study of the glial fibrillary acidic protein, Brain Res. 37: 81–89.PubMedCrossRefGoogle Scholar
  47. Uyemura, K., Vincendon, G., Gombos, G., and Mandel, P., 1971, Purification and some properties of S-100 protein fractions from sheep and pig brains, J. Neurochem. 18: 429–438.PubMedCrossRefGoogle Scholar
  48. Vincendon, G., Waksman, A., Uyemura, K., Tardy, J., and Gombos, G., 1967, Ultracentrifugal behavior of beef brain S-100 protein fraction, Arch. Biochem. 120: 233–235.PubMedCrossRefGoogle Scholar
  49. Warecka, K., 1970, Isolation of a brain specific glycoprotein, J. Neurochem. 17: 829–830.PubMedCrossRefGoogle Scholar
  50. Warecka, K., Moller, H. J., Vogel, H.-M., and Tripatzis, I., 1972, Human brain-specific alpha 2-glycoprotein</b>: Purification by affinity chromatography and detection of a new component ; Localization in nervous cells, J. Neurochem. 19: 719–725.Google Scholar
  51. Wasserman, E., and Levine, L., 1961, Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition, J. Immunol. 87: 290–295.PubMedGoogle Scholar
  52. Zomzely-Neurath, C, York, C., and Moore, B. W., 1972, Synthesis of a brain-specific protein (S-100 protein) in a homologous cell-free system programmed with cerebral polysomal messenger RNA, Proc. Natl. Acad. Sci. (U.S.) 69: 2326–2330.CrossRefGoogle Scholar
  53. Zomzely-Neurath, C, York, C, and Moore, B. W., 1973, In vitro synthesis of two brain-specific proteins (S-100 and 14–3–2) by polyribosomes from rat brain, Arch. Biochem. Biophys. 155: 58–69.PubMedCrossRefGoogle Scholar
  54. Zuckerman, J. E., Herschman, H. R., and Levine, L., 1970, Appearance of a brain specific antigen (the S-100 protein) during human foetal development, J. Neurochem. 17: 247–251.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1975

Authors and Affiliations

  • Blake W. Moore
    • 1
  1. 1.Department of PsychiatryWashington University School of MedicineSt. LouisUSA

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