Infectious Diseases

  • Dennis W. Ross


In the first three chapters we have considered the basic principles and technology that underlie molecular medicine. This chapter on infectious diseases begins part 2, in which we will apply recombinant DNA technology to the study, diagnosis, and treatment of disease. For infectious diseases, the application of molecular medicine is both straightforward and dramatic. We can take any clinical sample and analyze it for the presence of foreign, nonhuman DNA. The source of the foreign DNA can be identified as to genus and species of the invading infectious agent.


Human Immunodeficiency Virus Herpes Simplex Virus Human Papilloma Virus Infectious Agent Chlamydia Trachomatis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Barker RH, Brandling-Bennett AD, Koech DK, Mugambi M, Khan B, David R, David JR, Wirth DF (1989) Plasmodium falciparum: DNA probe diagnosis of malaria in Kenya. Exp Parasitol 69: 226–233.Google Scholar
  2. Dahlen PO, Iitia AJ, Skagius G, Frostell A, Nunn MF, Kwiatkowski M (1991) Detection of human immunodeficiency virus type 1 by using the polymerase chain reaction and a time-resolved fluroescence-based hybridization assay. J Clin Microbiol 29: 798–804.PubMedGoogle Scholar
  3. Eisenstein BI (1990) New molecular techniques for microbial epidemiology and the diagnosis of infectious diseases. J Infect Dis 161: 595–602.PubMedCrossRefGoogle Scholar
  4. Figueroa ME, Rasheed S (1991) Molecular pathology and diagnosis of infectious diseases. Am J Clin Pathol (Suppl) 95: S8 - S21.Google Scholar
  5. Granato PA, Franz MR (1989) Evaluation of a prototype DNA probe test for the noncultural diagnosis of gonorrhea. J Clin Microbiol 27: 632–635.PubMedGoogle Scholar
  6. Iwen PC, Tina MS, Blair MH, Woods GL (1991) Comparison of the Gen-Probe PACE 2TM system, direct fluorescent-antibody, and cell culture for detecting Chlamydia trachomatis in cervical specimens. Am J Clin Pathol 95: 578–582.PubMedGoogle Scholar
  7. Levy JA (1989) Human immunodeficiency viruses and the pathogenesis of AIDS. JAMA 261: 2997–3006.PubMedCrossRefGoogle Scholar
  8. McGowan KL (1989) Infectious diseases: diagnosis utilizing DNA probes. Clin Pediatr (Phila) 28: 157–162.CrossRefGoogle Scholar
  9. Munoz FJ, Sharon N (1990) Detection of human cytomegalovirus and Epstein-Barr Virus in peripheral blood mononuclear cells with DNA probes. Lab Med 21: 742–744.Google Scholar
  10. Ou CY, Kwok S, Mitchell SW, Mack DH, Sninsky JJ, Krebs JW, Feorino P, Warfield D, Schochetman G (1988) DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science 239: 295–297.PubMedCrossRefGoogle Scholar
  11. Panke ES, Yang LI, Leist PA, Magevney P, Fry RJ, Lee RF (1991) Comparison of Gen-Probe DNA probe test and culture for the detection of Neisseria gonorrhoeae in endocervical specimens. J Clin Microbiol 29: 883–888.PubMedGoogle Scholar
  12. Rivera MJ, Rivera N, Castillo J, Rubio MC, Gomez-Lus R (1991) Molecular and epidemiological study of Salmonella clinical isolates. J Clin Microbiol 29: 927–932.PubMedGoogle Scholar
  13. Schwarz TF, Nerlich A, Hottentrager B, Jager G, Wiest I, Kantimm S, Roggendorf H, Schultz M, Gloning K, Schramm T, Holzgreve W, Roggendorf M (1991) Parvovirus B19 infection of the fetus: histology and in situ hybridization. Am J Clin Pathol 96: 121–126.PubMedGoogle Scholar
  14. Sevell JS (1990) Detection of parvovirus B19 by dot-blot and ploymerase chain reaction. Mol Cell Probes 4: 237–246.CrossRefGoogle Scholar
  15. Tenover FC (1988) Diagnostic deoxyribonucleic acid probes for infectious diseases. Clin Microbiol Rev 1: 82–101.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Dennis W. Ross
    • 1
    • 2
  1. 1.The School of Medicine, Department of PathologyThe University of North Carolina at Chapel HillChapel HillUSA
  2. 2.Department of PathologyForsyth Memorial HospitalWinston-SalemUSA

Personalised recommendations