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Brain-Specific Migration and Protective Roles in Brain Damage of Microglia

A new therapeutic approach for catecholamine neuronal disfunctions
  • Makoto Sawada
  • Fumihiro Imai
  • Hiromi Suzuki
Part of the Advances in Behavioral Biology book series (ABBI, volume 53)

Abstract

The ability to manipulate the expression of genes within the mammalian brain provide unique opportunities to study and to potentially treat neurological disorders. The introduction of certain genes specifically in brain has been done with viral vectors or cells carrying DNA. However, these methods require surgery. Recently, we found that primary isolated microglia specifically entered the brain from blood flow when the cells were injected intra-arterially.1 Microglia, macrophage-like cells in the brain, are multi-functional cells; they play important roles in the development, differentiation and maintenance of neural cells via their phagocytic activity and production of enzymes, cytokines and trophic factors.2 Since intra-arterially-injected microglia were labeled with fluorescent dye microparticles by their phagocytic activity, this system could apply to a brain-specific delivery for medicines, or other bioactive materials, such as proteins or genes.3 To apply this brain-specific bio-targeting system for treatment of neurological disorders, vehicle microglia should migrate to the region of insult and not augment the insult. Therefore, we investigated migration of systemically injected microglia into the ischemic brain.

Keywords

Phagocytic Activity Brain Damage Quinolinic Acid Forebrain Ischemia Galactosidase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    F. Imai, M. Sawada, H. Suzuki, N. Kiya, M. Hayakawa, T. Nagatsu, T. Marunouchi, and T. Kanno, Migration activity of microglia and macrophages into rat brain, Neurosci. Lett., 237, 49–52 (1997).PubMedCrossRefGoogle Scholar
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    M. Sawada, A. Suzumura, and T. Marunouchi, Cytokine network in the central nervous system and its roles in growth and differentiation of glial and neuronal cells, Int. J. Dev. Neurosci., 13, 253–264 (1995).PubMedCrossRefGoogle Scholar
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    M. Sawada, F. Imai, H. Suzuki, M. Hayakawa, T. Kanno, and T. Nagatsu, Brain-specific gene expression by immortalized microglial cell-mediated gene transfer in the mammalian brain, FEBS Lett., 433, 37–40 (1998).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Makoto Sawada
    • 1
  • Fumihiro Imai
    • 2
  • Hiromi Suzuki
    • 3
  1. 1.PRESTO, Japan Science and Technology Corporation and Institute for Comprehensive Medical ScienceFujita Health UniversityToyoake, AichiJapan
  2. 2.Department of NeurosurgeryFujita Health UniversityToyoake, AichiJapan
  3. 3.Institute for Comprehensive Medical ScienceFujita Health UniversityToyoake, AichiJapan

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