Mutants and Variants of the Human Antidepressant-Sensitive Norepinephrine Transporter
The norepinephrine transporter (NET) is responsible for the rapid re-uptake of synaptically released NE. Substrate transport by neurotransmitter transporters (NTTs) is principially posible in both directions. In Na+- dependent transporters the normal transport direction is given by the Na+ gradient mainted by Na+/K+-ATPase. The ions Na+ and Cl- are cosubstrates of the NET and both ions are also needed for substrate (e.g., NE) transport and binding of NET inhibitors such as nisoxetine or the tricyclic antidepressant desipramine (DMI). In transfected cells overexpressing the NET, a channel-like mode of the NET has also been described.1 The NET transports the catecholamines NE, DA and epinephrine and amines like amphetamine, and the NET is a primary target of cocaine and of clinically important antidepressants (such as DMI, doxepine and reboxetine). The NET is not only expressed in noradrenergic neurons of the central and peripheral nervous system and in adrenal medullary cells but also in the placenta and in endothelial cells of small blood vessels of the lung where the NET is involved in the inactivation of circulating catecholamines.2
KeywordsSplice Variant Monoamine Transporter Norepinephrine Transporter Gaba Transporter Transfected HEK293 Cell
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