Abstract
Polyhydroxyalkanoates (PHA) are intracellular polymers stored by many bacterial species. Presently PHA are industrially produced by pure cultures fermentation where high quality substrates are used. Mixed cultuires using raw substrates are able to produce PHA when submitted to transient conditions like oscillations on substrate feeding or on oxygen supply. The yield on PHA produced by activated sludge submitted to these dynamic conditions reach values comparable to those obtained .by the pure cultures, being the first process less cost intensive than the last one. The chain length of the polymer produced in both processes is similar.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Anderson, A. J. and Dawes, E. A., 1990, Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev., 54: 450–472.
Page, W. and Cornish, A., 1993, Growth of Azotobacer vinelandii uwd in fish peptone medium and simplified extraction of poly-ß-hydroxybutyrate. Appl. Environ. Microbiol. 59: 4236–4244.
Lemos, P. C., Viana, C., Salgueiro, E. N., Ramos, A. M., Crespo, J. P. S. G. and Reis, M. A. M., 1998, Effect of carbon source on the formation of polyhydroxyalkanoates (PHA) by a phosphate-accumulating mixed culture. Enzyme and Microb. Technol. 22: 662–671.
Van Loosdrecht, M. C. M., Pot, M. A. and Heijen, J. J., 1997, Importance of bacterial storage polymers in bioprocesses. Wat. Sci. Technol. 35: 41–47.
Pereira, H., Lemos, P. C., Carrondo, M. J. T., Crespo, J. P. S. G., Reis, M. A. M. and Santos, H., 1996, Model for carbon metabolism in biological phosphorus removal processes based on in vivo 13C-NMR labelling experiments. Water Res. 30: 2128–2138.
Mino, T., Liu, W. T., Satoh, H. and Matsuo, T., 1996, Possible metabolisms of polyphosphate accumulating (PAOs) and glycogen accumulating non-poly-P organisms (GAOs) in the enhanced biological phosphate removal process. Med. Fac. Landbouww. Univ. Gent. 61: 1769–1775.
Wentzel, M. C., Lotter, L. H., Ekama, G. A., Loewenthal, R. E. and Marais, G. V., 1991, Evaluation of biochemical models for biological excess phosphorus removal. Wat. Sci. Technol. 23: 567–576.
Satoh, H., Mino, T. and Matsuo, T., 1992, Uptake of organic substrates and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in the biological excess phosphate removal processes. Wat. Sci. Technol., 26: 933–942.
Rees, G. N., Vasilidis, G., May, J. W. and Bayly, R. C., 1992, Differentiation of polyphosphate and poly-ß-hydroxybutyrate granules in an Acinetobacter sp. isolated from activated sludge. Lett. Appl. Micro., 25: 63–69.
Satoh, H., Iwamoto, Y., Mino, T. and Matsuo, T., 1998, Activated sludge as a possible source of biodegradable plastic. Wat. Sci. Technol., 38: 103–109.
Lee, S. Y., 1996, Bacterial polyhydroxyalkanoates. Biotechnol. Bioeng., 49: 1–14.
Akiyama, M., Taima, Y. and Doi, Y., 1992, Production of poly(3-hydroxyalkanoates) by a bacterium of the genus Alcaligenes utilising long-chain fatty acids. Appl. Microbiol. Biotechnol., 37: 698–701.
Shimizu, H., Tamura, S., Shioya, S. and Suga, K., 1993, Kinetic study of poly-D(-)-3-hydroxybutyric acid (PHB) production and its molecular weight distribution control in a fed-batch culture of Alcaligenes eutrophus. J. Ferment. Bioeng., 76: 465–469.
Taidi, B., Mansfield, D. and Anderson, A., 1995, Turnover of poly(3-hydroxybutyrate) (PHB) and its influence on the molecular mass of the polymer accumulated by Alcaligenes eutrophus during batch culture. FEMS Microbiol. Lett., 120: 201–206.
Taidi, B., Anderson, A. J., Dawes, E. A. and Byron, D., 1994, Effect of carbon source and concentration on the molecular mass of poly(3-hydroxybutyrate) produced by Methylobacterium extorquens and Alcaligenes eutrophus. Appl. Environ. Microbiol., 40: 786–790.
Hiramitsu, M., Koyama, N. and Doi, Y., 1993, Production of poly(3-hydroxybutyrare-co-4-hydroxybutyrate) by Alcaligenes latus. Biotechnol. Lett., 15: 461–464.
Chen, G. and Page, W., 1994, The effect of substrate on the molecular weight of poly-ß-hydroxybutyrate produced by Azotobacter vinelandii uwd. Biotechnol. Lett. 16: 155–160.
Daniel, M., Choi, J., Kim, J. and Lebeault, J., 1992, Effect of nutrient deficiency on accumulation and relative molecular weight of poly-ß-hydroxybutyric acid by methylotrophic bacterium, Pseudomonas 135. Appl. Microbiol. Biothecnol., 37: 702–706.
Young, F., Kastner, J. and May, S., 1994, Microbial production of PHB from D-xylose and lactose by Pseudomonas cepacia. Appl. Environ. Microbiol., 60: 4195–4198.
Anderson, A., Williams, D., Taidi, B., Dawes, E. and Ewing, D., 1992, Studies on copolyester synthesis by Rhodococus ruber and factors influencing the molecular mass of polyhydroxybutyrate accumulated by methylobacterium extorquens and Alcaligenes eutrophus. FEMS Microbiol. Rev., 103: 93–102.
Beccari, M., Majone, M., Ramadori, R. and Tozzi, G., 1997, Biodegradable polymers from wastewater treatment by using selected mixed cultures. XII Convegno Div. Chim. Ind. E Cruppu Interdiv. Catalisi, SCI, Giarden, Italy, 22–25 July .
Majone, M., Dircks, K. and Beun, J. J., 1999, Aerobic storage under dynamic conditions in activated sludge processes. The state of the art. Wat. Sci. Technol., 39: 61–73.
Majone, M., Massanisso, P. and Ramadori, R., 1998, Comparison of carbon storage under aerobic and anoxic conditions. Wat. Sci. Technol., 38: 77–84.
Majone, M., Massanisso, P., Carucci, A., Lindrea, K. and Tandoi, V., 1996, Influence of storage on kinetic selection to control aerobic filamentous bulking. Wat. Sci. Technol., 34: 223–232.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media New York
About this chapter
Cite this chapter
Serafim, L.S., Lemos, P.C., Crespo, J.G., Ramos, A.M., Reis, M.A.M. (2001). Polyhydroxyalkanoates Production by Activated Sludge. In: Chiellini, E., Gil, H., Braunegg, G., Buchert, J., Gatenholm, P., van der Zee, M. (eds) Biorelated Polymers. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-3374-7_15
Download citation
DOI: https://doi.org/10.1007/978-1-4757-3374-7_15
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-3369-0
Online ISBN: 978-1-4757-3374-7
eBook Packages: Springer Book Archive