DNA Repair and Simple Recombination

  • Edward A. Birge


All organisms and some viruses have their own mechanisms for maintaining the integrity of their nucleic acid (i.e., for repairing any damage). Nevertheless, most organisms can undergo some sort of genetic transfer or exchange process(es). The two mechanisms may seem antithetical because recombination, the movement of genetic information from one molecule of nucleic acid to another, implies that a nucleic acid molecule loses its integrity and undergoes some kind of structural alteration. However, as is discussed in this chapter, one way to view the recombination process is that it has appropriated the essential DNA repair processes for a function in which the potential for damage to the genetic information contained in a nucleic acid molecule is outweighed by the potential benefit to be derived from new genetic information. Viewed in this way, the genetic transfer processes that are discussed in subsequent chapters are really afterthoughts that trigger preexisting repair pathways to accomplish DNA recombination.


RecA Protein Pyrimidine Dimer Strand Exchange Holliday Junction Nucleic Acid Molecule 


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  1. Adams, R.L.P., Knowler, J.T., Leader, D.P. (1992). The Biochemistry of the Nucleic Acids, 11 th ed. London: Chapman & Hall. (Chapter 7 discusses repair and recombination.)Google Scholar
  2. Friedberg, E. C., Walker, G.C., Siede, W. (1995). DNA Repair and Mutagenesis. Washington, DC: ASM Press.Google Scholar
  3. Kanai, S., Kukuno, R., Toh, H., Ryo, H., Todo, T. (1997). Molecular evolution of the photolyase-blue-light photoreceptor family. Journal of Molecular Evolution 45: 535–548.PubMedCrossRefGoogle Scholar
  4. Radding, C.M. (1993). A universal recombination filament. Current Biology 3: 358–360.Google Scholar
  5. Roca, A.I., Cox, M.M. (1997). RecA protein: Structure, function, and role in recombinational DNA repair. Progress in Nucleic Acid Research and Molecular Biology 56: 129–223.CrossRefGoogle Scholar
  6. Smith, G.R., Amundsen, S.K., Dabert, P., Taylor, A.F.(1995). The initiation and control of homologous recombination in Escherichia coli. Philosophical Transactions of tbe Royal Society of London, Series B 347: 13–20.CrossRefGoogle Scholar
  7. Van Houten B. (1990). Nucleotide excision repair in Escherichia coli. Microbiological Reviews 54: 18–51. (Detailed molecular biology of the UvrABC nuclease complex.)PubMedGoogle Scholar
  8. Walker, G.C. (1998). Skiing the black diamond slope: Progress on the biochemistry of translesion DNA synthesis. Proceedings of tbe National Academy of Sciences of tbe USA 95: 10348–10350.CrossRefGoogle Scholar
  9. West, S.C. (1997). Processing of recombination intermediates by the RuvABC proteins. Annual Review of Genetics 31: 213–244.PubMedCrossRefGoogle Scholar


  1. Amundsen, S.K., Taylor, A.F., Smith, G.R. (1998). A stimulatory RNA associated with RecBCD enzyme. Nucleic Acids Research 26: 2125–2131.PubMedCrossRefGoogle Scholar
  2. Anderson, D.G., Kowalczykowski, S.C. (1998) SSB protein controls RecBCD enzyme nuclease activity during unwinding: A new role for looped intermediates. Journal of Molecular Biology 282: 275–285.PubMedCrossRefGoogle Scholar
  3. Bazemore, R., Folta-Stogniew, E., Takahashi, M., Radding, C.M. (1997). RecA tests homology at both pairing and strand exchange. Proceedings of the National Academy of Sciences of the USA 94: 1 1 863–1 1 868.CrossRefGoogle Scholar
  4. Chiu, S.-K., Low, K.B., Yuan, A., Radding, C.M. (1997). Resolution of an early RecA-recombination intermediate by a junction-specific endonuclease. Proceedings of the National Academy of Sciences of the USA 94: 6079–6083.CrossRefGoogle Scholar
  5. Fijalkowska, I.J., Jonczyk, P., Tkaczyk, M.M., Bialoskorska M., Schaaper, R.M. (1998). Unequal fidelity of leading strand and lagging strand DNA replication on the Escherichia coli chromosome. Proceedings of the National Academy of Sciences of the USA 95: 10020–10025.PubMedCrossRefGoogle Scholar
  6. Friedman-Ohana, R., Karunker, I., Cohen, A. (1998). Chi-dependent intramolecular recombination in Escherichia coli. Genetics 148: 545–557.Google Scholar
  7. Gupta, R.C., Golub, E.I., Wold, M.S., Radding, C.M. (1998). Polarity of DNA strand exchange promoted by recombination proteins of the RecA family. Proceedings of the National Academy of Sciences of the USA 95: 9843–9848.PubMedCrossRefGoogle Scholar
  8. Li, S., Waters, R. (1998). Escherichia coli strains lacking protein HU are UV sensitive due to a role for HU in homologous recombination. Journal of Bacteriology 180: 3750–3756.PubMedGoogle Scholar
  9. Liu, Y. -H., Cheng, A. -J.. Wang. T.-C. V. (1998). Involvement of recF, recO, and recR genes in UIV radiation mutagenesis of Escherichia coli. Journal of Bacteriology 180: 1766–1770.Google Scholar
  10. Reuven, N.B., Tomer, G., Livneh, Z. (1998). The mutagenesis proteins UImuD’ and UmuC prevent lethal frameshifts while increasing base substitution mutations. Molecular Cell 2: 191–199.PubMedCrossRefGoogle Scholar
  11. Seitz, E.M., Brockman, J.P., Clark, A.J., Kowalczykowski, S.C. (1998). RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange. Genes & Development 12: 1248–1253.CrossRefGoogle Scholar
  12. Tambuk, S., Radman, M. (1998). Mechanism and control of interspecies recombination in Escherichia coli. I. Mismatch repair, methylation, recombination, and replication functions. Genetics 150: 533–542.Google Scholar
  13. Tang, M., Bruck, I., Eritia, R., Turner, J., Frank, E., Woodgate, R., O’Donnell, M., Goodman, M.F. (1998). Biochemical basis of SOS-induced mutagenesis in Escherichia coli: Reconstitution of in vitro lesion bypass dependent on the UmuD’2C mutagenic complex and RecA protein. Proceedings of the National Academy of Sciences of the USA 95: 9755–9760.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Edward A. Birge
    • 1
  1. 1.Department of MicrobiologyArizona State UniversityTempeUSA

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