Plasmids and Conjugation Systems Other Than F

  • Edward A. Birge


Not all plasmids are conjugative, and even those that are do not necessarily have chromosome-mobilizing ability (i.e., the ability to promote transfer of chromosomal markers to a recipient cell). Nevertheless, these plasmids can have major economic and genetic importance. The diversity of plasmids that have been identified is staggering, and they are ubiquitous. In one study, 34 of 87 hospital isolates of enteric bacteria or Pseudomonas carried at least one plasmid. Bacillus megaterium routinely has eight or more plasmids in its cytoplasm. All of these plasmids have only two things in common. They can be identified in cell lysates as autonomous DNA molecules, and they are capable of self-replication. If the presence or absence of a plasmid has no observable effect on the cell phenotype, it is a cryptic plasmid. Researchers often subdivide plasmids according to their capacity for self-transfer from one host cell to another using the F plasmid as the standard for comparison.


Tetracycline Resistance Conjugation System Conjugative Plasmid Incompatibility Group Resistance Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.



  1. Clewell, D.B. (ed.) (1993). Bacterial Conjugation. New York: Plenum Press. (Included are chapters on Ti plasmids, R100, conjugative transposition, and pheromone effects.)Google Scholar
  2. Holloway, B.W. (1998). The less traveled road in microbial genetics. Microbiology 144: 3243–3248.PubMedCrossRefGoogle Scholar
  3. Kado, C.I. (1998). Origin and evolution of plasmids. Antonie van Leeuwenhoek 73: 117–126. (Includes a good discussion of the Ti plasmid.)PubMedCrossRefGoogle Scholar
  4. Lazdunski, C.J., Bouveret, E., Rigal, A., Journet, L., Lloubès, R., Bénédetti (1998). Colicin import into Escherichia coli cells. Journal of Bacteriology180: 4993–5002.PubMedGoogle Scholar
  5. Luria, S.E., Suit, J.L (1987). Colicins and Col plasmids, pp. 1615–1624. In: Neidhardt, F.C. et al. (eds.), Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. Washington, DC: American Society for Microbiology.Google Scholar
  6. Meijer, W.J.J., Wisman, G.B.A., Terpstra, P, Thorsted, PB., Thomas, C.M., Holsappel, S., Venema, G., Bron, S. (1998). Rolling-circle plasmids from Bacillus subtilis: Complete nucleotide sequences and analyses of genes of pTA1015, pTA1040, pTA1050 and pTA1060, and comparisons with related plasmids from gram-positive bacteria. FEMS Microbiology Reviews 21: 337–368.PubMedCrossRefGoogle Scholar
  7. Noll, K.M., Vargas, M. (1997). Recent advances in genetic analyses of hyperthermophilic Archaea and Bacteria. Archives of Microbiology168: 73–80.CrossRefGoogle Scholar
  8. O’Brien, S.J. (1993). Genetic Maps, 6th ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.Google Scholar
  9. Prangishvili, D., Albers, S.-V., Holz, I., Arnold, H.P., Stedman, K., Klein, T., Singh, H., Hiort, J., Schweier, A., Kristjansson, J.K., Zillig, W. (1998). Conjugation in Archaea: Frequent occurrence of conjugative plasmids in Sulfolobus. Plasmid 40: 190–202.CrossRefGoogle Scholar
  10. Scott, J.R. (1992). Sex and the single circle: Conjugative transposition. Journal of Bacteriology 174: 6005–6010.PubMedGoogle Scholar
  11. Zatyka, M., Thomas, C.M. (1998). Control of genes for conjugative transfer of plasmids and other mobile elements. FEMS Microbiology Reviews 21: 291–319.CrossRefGoogle Scholar


  1. Bohne, J., Yim, A., Binns, A.N. (1998). The Ti plasmid increases the efficiency of Agrobacterium tumefaciens as a recipient in virB-mediated conjugal transfer of an IncQ plasmid. Proceedings of the National Academy of Sciences of the USA 95: 7057–7062.PubMedCrossRefGoogle Scholar
  2. Celli, J., Trieu-Cuot, P. (1998). Circularization of Tn916 is required for expression of the transposon-encoded transfer functions: characterization of long tetracycline-inducible transcripts reading through the attachment site. Molecular Microbiology 28: 103–117.PubMedCrossRefGoogle Scholar
  3. Lee, S.H., Frost, L.S., Paranchych, W. (1992). FinOP repression of the F plasmid involves extension of the half-life of FinP antisense RNA by FinO. Molecular and General Genetics 235: 131–13 9.Google Scholar
  4. Marra, D., Scott, J.R. (1999). Regulation of excision of the conjugative transposon Tn916. Molecular Microbiology 31: 609–621.PubMedCrossRefGoogle Scholar
  5. Nakayama, J., Takanami, Y., Horii, T., Sakuda, S., Suzuki, A. (1998). Molecular mechanism of peptide-specific pheromone signaling in Enterococcus faecalis: Functions of pheromone receptor TraA and pheromone-binding protein TraC encoded by plasmid pPD1. Journal of Bacteriology 180: 449–456.PubMedGoogle Scholar
  6. Redenbach, M., Kieser, H.M., Denapaite, D., Eichner, A., Cullum, J., Kinashi, H., Hopwood, D.A. (1996). A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome. Molecular Microbiology. 21: 77–96.PubMedCrossRefGoogle Scholar
  7. Zhu, J., Winans, S.C. (1998). Activity of the quorum-sensing regulator TraR of Agrobacterium tumefaciens is inhibited by a truncated, dominant defective TraR-like protein. Molecular Microbiology 27: 289–297.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Edward A. Birge
    • 1
  1. 1.Department of MicrobiologyArizona State UniversityTempeUSA

Personalised recommendations