During the past 12 years, there have been remarkable advances in the use of fluorescence to study DNA. Fluorescence methods are now used for DNA sequencing, detection of DNA hybridization and restriction enzyme fragments, fluorescence in situ hybridization (FISH), and quantitating polymerase chain reaction products. Because of the rapid introduction of new DNA technology, it is surprising to realize that DNA sequencing by fluorescence was first reported just 12 years ago, in 1986. It is not the purpose of this chapter to describe the many specialized methods used in this extensive area of molecular biology and diagnostics. Instead, we give a brief introduction to each topic, followed by a description of the unique fluorophores and principles used for each application.


Fluorescent Primer Thiazole Orange Pulse Laser Diode Thiazole Orange Pyrene Monomer 


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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Joseph R. Lakowicz
    • 1
  1. 1.University of Maryland School of MedicineBaltimoreUSA

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