Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers
In the previous chapter we described the principles of resonance energy transfer and how the phenomenon could be used as a “spectroscopic ruler” to measure distances between donor and acceptor sites on macromolecules. Energy transfer was described as a through-space interaction which occurred whenever the emission spectrum of the donor overlapped with the absorption spectrum of the acceptor. For a given donor—acceptor (D—A) pair, the efficiency of energy transfer decreases as r −6, where r is the D—A distance. Each D—A pair has a characteristic distance, the Förster distance (R 0), at which RET is 50% efficient. The extent of energy transfer, as seen from the steady-state data, can be used to measure the distance, to determine the extent of association based on proximity, or to determine the distance of closest approach between the D—A pair.
KeywordsEnergy Transfer Fluorescence Resonance Energy Transfer Distance Distribution Intensity Decay Bovine Pancreatic Trypsin Inhibitor
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