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Time-Dependent Anisotropy Decays

  • Joseph R. Lakowicz

Abstract

In the preceding chapter we described the measurement and interpretation of steady-state fluorescence anisotropies. These values are measured using continuous illumination and represent an average of the anisotropy decay over the intensity decay. Measurement of steady-state anisotropies is simple, but interpretation of the steady-state anisotropies usually depends on an assumed form for the anisotropy decay, which is not directly observed in the experiment. Additional information is available if one measures the time-dependent anisotropy, that is, the values of r(t) following pulsed excitation. The form of the anisotropy decay depends on the size, shape, and flexibility of the labeled molecule, and the data can be compared with the decays calculated from various molecular models. Anisotropy decays can be obtained using the TD or the FD method.

Keywords

Correlation Time Yellow Fluorescent Protein Fluorescence Anisotropy Rotational Diffusion Intensity Decay 
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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Joseph R. Lakowicz
    • 1
  1. 1.University of Maryland School of MedicineBaltimoreUSA

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