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Chemiluminescence Measurements for the Detection of Free Radical Species

  • A. Süha Yalçın
  • Goncagül Haklar
  • Belgin Küçükkaya
  • Meral Yüksel
  • Gönül Dalaman
Chapter
Part of the NATO ASI Series book series (NSSA, volume 296)

Abstract

Reactive oxygen species (ROS) have been implicated in the pathogenesis of a variety of human diseases (Halliwell and Gutteridge, 1989). Increased appreciation of occurence of ROS, their injury potential and pathogenic role in several disease states demands quantitative methods which are diagnostic of the process and meet basic analytical criteria regarding accuracy, reliability, sensitivity and specificity (Halliwell and Grootveld, 1987). However, because of their reactive nature and short half lives, it is hard to quantitate ROS. Instead analyses of secondary or end products produced by their attack on lipids, proteins or other cellular components are preferred. These indirect methods usually give misleading results due to their poor specificity and sensitivity.

Keywords

Acute Pancreatitis Reactive Oxygen Species Formation Infertile Patient Cumene Hydroperoxide Free Radical Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • A. Süha Yalçın
    • 1
  • Goncagül Haklar
    • 1
  • Belgin Küçükkaya
    • 1
  • Meral Yüksel
    • 1
  • Gönül Dalaman
    • 1
  1. 1.Department of BiochemistryFaculty of Medicine Marmara UniversityIstanbulTurkey

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