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Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF)-Deficient Mice

  • Ashley R. Dunn
  • Graham J. Lieschke
Chapter
Part of the Contemporary Immunology book series (CONTIM)

Abstract

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a secreted glycoprotein hemopoietic growth factor (124 amino acids in mouse and 127 amino acids in human) that in vitro stimulates the survival, proliferation, and function of myeloid cells (For review, see ref. 1). GM-CSF has a broad range of actions on target cells that include neutrophils, eosinophils, macrophages, erythroid progenitors, megakaryocyte progenitors, and antigen-presenting dendritic cells (For review, see ref. 2). The potency of GM-CSF in vivo has been demonstrated by generating GM-CSF transgenic mice (3) and by reconstituting mice with marrow cells previously infected with a recombinant retrovirus expressing GM-CSF (4). The hemopoietic system of mice exposed to excess levels of GM-CSF in these ways is profoundly affected with increases in both progenitor cells and granulocytes and macrophages. These animal models suggest that GM-CSF excess results in a myeloproliferative syndrome, but this does not lead to leukemia. However, preleukemic growth factor-dependent clones infected with recombinant retrovirus encoding GM-CSF are able to proliferate in the absence of exogenous GM-CSF and are uniformly leukemogenic in the syngeneic mouse (5). To establish the normal physiological role of GM-CSF we (6) and others (7) generated GM-CSF deficient mice by gene targeting in embryonic stem cells.

Keywords

Deficient Mouse Lamellar Body Pulmonary Alveolar Proteinosis Pulmonary Pathology Lymphoid Infiltration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Ashley R. Dunn
  • Graham J. Lieschke

There are no affiliations available

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