DNA Amplification Techniques

An Overview
  • David H. Sorscher
Part of the Pathology and Laboratory Medicine book series (PLM)


The polymerase chain reaction (PCR) has revolutionized modern science. With the introduction of the PCR to the clinical laboratory, genetically based diseases are now diagnosed with an incredible level of sensitivity and accuracy. This chapter will review the original discovery of the PCR in which the synthetic cycles were driven by the Klenow firagment of DNA polymerase I, and the rapid development of this exciting technology after the introduction of the heat-resistant Thermus aquaticus (Taq) polymerase.


Polymerase Chain Reaction Capture Probe Duchenne Muscular Dystrophy Nucleic Acid Sequence Base Amplification Ligase Chain Reaction 


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© Springer Science+Business Media New York 1997

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  • David H. Sorscher

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