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Microexplant Cultures of the Cerebellum

  • Bernard Rogister
  • Gustave Moonen

Abstract

This chapter is devoted to a simple method for culturing developing rat cerebellum. The method can also be used for rat hippocampus embryonic age 18 (E18), cerebral cortex (E18), and even spinal cord, but at an earlier stage of development (E14). This method was initially designed to obtain long-term survival of cerebellar macroneurons, Purkinje cells, and deep nuclear macroneurons (Moonen et al., 1982; Neale et al., 1982). Indeed, in cultures in which a single cell suspension is seeded, virtually all the neurons die between 5 and 10 d, whereas significant survival is obtained if small aggregates of cells (microexplants) rather than single cells are seeded. The term “microexplant” was used to stress the difference from the classic cerebellar expiants that consist of a thin cerebellar slice, which are much more “organized” tissue samples.

Keywords

Purkinje Cell Minimum Essential Medium Cerebellar Granule Cell Laminar Flow Hood Radial Glial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Further Reading

  1. Cull-Candy, S. G. and Usowicz, M. M. (1987), Multiple-conductance channels activated by excitatory amino acids in cerebellar neurons. Nature 325, 525–528.PubMedCrossRefGoogle Scholar
  2. Eagle, H. (1959), Amino acid metabolism in mammalian cell cultures. Science 130, 432–437.PubMedCrossRefGoogle Scholar
  3. Fischer, G., Kunemund, V., and Schachner, M. (1986), Neurite outgrowth patterns in cerebellar microexplant cultures are affected by antibodies to the cell surface glycoprotein L1. J. Neurosci. 6, 605–612.PubMedGoogle Scholar
  4. Gibbs, W., Neale, E. A., and Moonen, G. (1982), Kainic acid sensitivity of mammalian Purkinje cells in monolayer cultures. Dev. Br. Res. 4, 103–108.CrossRefGoogle Scholar
  5. Grau-Wagemans, M.-P., Selak, I., Lefebvre, P. P., and Moonen, G. (1984), Cerebellar macroneurons in serum-free cultures. Evidence for intrinsic neuronotrophic and neuronotoxic activities. Dev. Br. Res. 15, 11–19.CrossRefGoogle Scholar
  6. MacDonald, R. L., Moonen, G., Neale, E. A., and Nelson, P. G. (1982), Cerebellar macroneurons in microexplant cell culture. Postsynaptic amino acid pharmacology. Dev. Br. Res. 5, 75–88.CrossRefGoogle Scholar
  7. Moonen, G., Neale, E. A., MacDonald, R. L., Gibbs, W., and Nelson, P. G. (1982), Cerebellar macroneurons in microexplant cell culture. Methodology, basic electrophysiology, and morphology after horseradish peroxidase injection. Dev. Br. Res. 5, 59–73.CrossRefGoogle Scholar
  8. Neale, E. A., Moonen, G., MacDonald, R. L., and Nelson, P. G. (1982), Cerebellar macroneurons in microexplant cell cultures: ultrastructural morphology. Neuroscience 7, 1879–1890.PubMedCrossRefGoogle Scholar
  9. Selak, I., Foidart, J. H., and Moonen, G. (1985), Laminin promotes cerebellar granule cells migration in vitro and is synthesized by cultured astrocytes. Dev. Neurosci. 7, 278–285.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Bernard Rogister
  • Gustave Moonen

There are no affiliations available

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