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Tips in Tissue Culture

  • Arleen Richardon
  • Sergey Fedoroff

Abstract

Good aseptic technique is superior to the use of antibiotics in tissue culture. In certain cases, however, the prophylactic use of antibiotics is invaluable. Antibiotics should be used when the starting material for primary cultures is not sterile, e.g., tissue obtained from the abattoir, human tissue obtained during surgery or postmortem, and tissue from nasal passages, gastrointestinal and urogenital tract, and skin. In such cases, the tissue should be bathed in a solution containing a high concentration of antibiotics before initiating cultures. Antibiotics can also be used in the cultures, but for as short a period of time as possible, up to a maximum of 2 wk of culturing. Antibiotics can also be used prophylactically in experiments in which the cultures are terminated at the end of the experiment. In exceptional cases, antibiotics may be used to decontaminate irreplaceable cultures (see below).

Keywords

Water Purification System Laminar Flow Hood Fungal Contamination Streptomycin Solution Tissue Culture Laboratory 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Further Reading

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Arleen Richardon
  • Sergey Fedoroff

There are no affiliations available

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