Good aseptic technique is superior to the use of antibiotics in tissue culture. In certain cases, however, the prophylactic use of antibiotics is invaluable. Antibiotics should be used when the starting material for primary cultures is not sterile, e.g., tissue obtained from the abattoir, human tissue obtained during surgery or postmortem, and tissue from nasal passages, gastrointestinal and urogenital tract, and skin. In such cases, the tissue should be bathed in a solution containing a high concentration of antibiotics before initiating cultures. Antibiotics can also be used in the cultures, but for as short a period of time as possible, up to a maximum of 2 wk of culturing. Antibiotics can also be used prophylactically in experiments in which the cultures are terminated at the end of the experiment. In exceptional cases, antibiotics may be used to decontaminate irreplaceable cultures (see below).
KeywordsWater Purification System Laminar Flow Hood Fungal Contamination Streptomycin Solution Tissue Culture Laboratory
Unable to display preview. Download preview PDF.
- (1992), Testing for microbial contaminants, in ATCC Quality Control Methods for Cell Lines, Hay, R. J., Caputo, J., and Macy, M. L., eds., American Type Culture Collection, Rockville, MD, pp. 19–48.Google Scholar
- Fogh, J., ed. (1973), Contamination in Tissue Culture. Academic, New York, pp. 1–288.Google Scholar
- Goule, M. C. (1984), Endotoxin in vertebrate cell culture: its measurement and significance, in Uses and Standardization of Vertebrate Cell Cultures, Tissue Culture Association, MD 5, 125–136.Google Scholar
- Mather, J., Kaczarowski, F., Gabler, R., and Wilkins, F. (1986), Effects of water purity and addition of common water contaminants on the growth of cells in serum-free media. BioTechniques 4, 56–63.Google Scholar
- Perkins, J. J. (1969), Principles and Methods of Sterilization in Health Sciences. Charles C., Thomas, Springfield, IL, pp. 1–549.Google Scholar
- Perlman, D. (1979), Use of Antibiotics in cell culture media, in Methods in Enzymology: Cell Culture, vol. 58, Jacoby, W. B. and Pasten, I. H., eds., Academic, New York, pp. 110–116.Google Scholar
- Ryan, J. A. (1994), Understanding and managing cell culture contamination. Corning, Inc. Technical Publication TC-CI-559, Corning, NY.Google Scholar
- Taylor, W. G. (1984), Toxicity and hazards to successful culture: cellular responses to damage induced by light, oxygen or heavy metals, in Uses and Standardization of Vertebrate Cell Cultures, Patterson, M. K., ed., Tissue Culture Association, MD, 58–70.Google Scholar