Preparation of Tissue-Cultured Material for Electron Microscopical Observation
Electron microscopy is an invaluable tool that has enabled investigators to visualize, at high resolution, the fine structure of cells. Many steps are required in preparing tissue for electron microscopy; these include fixation, dehydration, embedding (infiltration and polymerization), mounting, and sectioning, as well as collection and staining of the sections. At each step, it is possible to introduce artifacts that affect the end results. Therefore, an understanding of the rationale for performing each step (i.e., rates of penetration for various types of fixatives, buffer combinations, infiltration rates, and temperatures used) will assist the investigator in preventing, or at least reducing, the number of possible artifacts that can cause the deterioration of the final image obtained by the electron microscope.
KeywordsToluidine Blue Osmium Tetroxide Potassium Ferricyanide Sodium Cacodylate Succinic Anhydride
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