Procedures for Subculturing Cells

  • Arleen Richardson
  • Sergey Fedoroff


Cells attach to the culture substratum with varying degrees of adherence. Some types of cells do not adhere at all and grow as cell suspensions in the medium. Such cells are referred to as nonadhering cells (nonanchorage-dependent cells). Cells that have to attach to the substratum to proliferate are referred to as adhering cells (anchorage-dependent cells).


Culture Vessel Disodium Salt Soybean Trypsin Inhibitor Nonadhering Cell Rubber Policeman 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Further Reading

  1. Bottenstein, J. , Hayashi, I., Hutchings, S., Masui, J., Mather, J., McClure, D. B., Ohasa, S., Rizzino, A., Sato, G., Serrero, G., Wolfe, R., and Wu, R. (1979), The growth of cells in serum-free hormone-supplemented media, in Methods in Enzymology Cell Culture, Jakoby, W. B. and Pastan, I. H., eds., Academic, New York, pp. 94–109.Google Scholar
  2. Melnick, J. L. and Wallis, C. (1977), Problems related to the use of serum and trypsin in the growth of monkey kidney cells, in Developments in Biological Standardization, Perkins, F. T. and Regamey, R. H., eds., S. Karger, Basel, pp. 77–82.Google Scholar
  3. McKeehan, W. L. (1977), The effect of temperature during trypsin treatment on viability and multiplication of single normal human and chicken fibroblasts. Cell Biol. Int. Reports 1, 335–343.CrossRefGoogle Scholar
  4. Waymouth, C. (1982), Methods for obtaining cells in suspension from animal tissues, in Cell Separation Methods and Selected Applications, vol. 1, Pretlow, T. G. and Pretlow, T. P., eds., Academic, New York, 1, 1–29.Google Scholar
  5. Waymouth, C. (1993), Tissue Dissociation Guide, Worthington Biochemical Corporation, Freehold, NJ, pp. 1–78.Google Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Arleen Richardson
  • Sergey Fedoroff

There are no affiliations available

Personalised recommendations