Colony cultures are based on the assumption that single viable cells can attach to the substratum, divide, and form a progeny of cells that constitute a cell colony. Colony cultures can be initiated either from the original disaggregated cell suspension made directly from animal tissue, or from primary or secondary cultures. When large numbers of viable cells are plated/cm2 of substratum, on culturing, a confluent layer of cells forms. When a small number of cells are plated/cm2 of substratum, then single, discrete cell colonies form. Colonies, especially ones initiated directly from tissues, are not necessarily uniform. The morphology and size of the colony depends on the kinds of cells plated, the degree of their differentiation, the cell generation time, the composition of the medium, the type of substratum, and the physical conditions. In practice, disaggregated tissue yields a variety of cells of varying degrees of maturity, resulting in heterogeneity in colony size and morphology. Colony cultures from secondary cultures usually have more uniform colonies.
KeywordsGlial Fibrillary Acidic Protein Culture Vessel Newborn Mouse Plating Efficiency Sodium Metasilicate
Unable to display preview. Download preview PDF.
- Fedoroff, S. (1984), A method for the study of neural cell lineages based on colony culture and transplantation of cultured cells into the CNS, in Developmental Neuroscience: Physiological, Pharmacological and Clinical Aspects, Caciagle, F., Giacobini, E., Paoletti, R., eds., Elsevier, Amsterdam, pp. 373–376.Google Scholar
- Ham, R. G. (1972), Cloning of mammalian cells. Methods Cell Physiol. 5, 37–74.Google Scholar
- Labourdette, G. and Sensenbrenner, M. (1995), Growth factors and their receptors in the central nervous system, in Neuroglia, Kettenman, H. and Ransom, B. R., eds., Oxford University Press, New York, pp. 441–459.Google Scholar
- Miller, R. H. and Szigeti, B. (1991), Clonal analysis of astrocyte diversity in neonatal rat spinal cord cultures. Deuel. 113, 353–362.Google Scholar
- Skoff, R. P. and Knapp, P. E. (1995), The origin and lineage of macroglial cells, in Neuroglia, Kettenmann, H. and Ransom, B. R., eds., Oxford University Press, New York, pp. 135–148.Google Scholar