The modern surgical pathology laboratory is equipped to perform a staggering number of routine and special diagnostic procedures. Most are carried out by the laboratory technologist; however, gross room personnel should be familiar with the basic concepts and initial steps of these procedures. Failure to handle tissue appropriately may preclude the performance of needed diagnostic studies and ultimately delay or even prevent the establishment of a diagnosis. This chapter provides a basic introduction to the more common techniques employed in tissue fixation, staining, decalcification, and intraoperative consultation.
KeywordsPicric Acid Mercuric Chloride Approximate Time Special Stain Ancillary Test
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- Culling CFA. Handbook of Histopathological and Histochemical Techniques. 3rd ed. Boston, Mass: Butter-worths and Co, Ltd; 1974.Google Scholar
- Hopwood D. Fixation and fixatives. In: Bancroft JD, Stevens A, eds. Theory and Practice of Histological Techniques. New York, NY: Churchill Livingstone; 1977: 16–28.Google Scholar
- Luna LG, ed. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed. New York, NY: McGraw-Hill Book Co; 1968.Google Scholar
- Naish SJ, Boenisch T, Farmilo AJ, Stead RH, eds. Handbook. Immunochemical Staining Methods. Carpinteria, Calif: DAKO Corp; 1989.Google Scholar
- Pearse AGE. Histochemistry. Theoretical and Applied. 2nd ed. Boston, Mass: Little, Brown, and Co, 1960.Google Scholar
- Sheehan DC, Hrapchak BB. Fixation. In: Sheehan DC, Hrapchak, BB, eds. Theory and Practice of Histotechnology. 2nd ed. St Louis, Mo: CV Mosby Co; 1980; 40–58.Google Scholar
- Therkildsen MH, Pilgaard J. Microwave-assisted frozen section diagnosis: a comparison between conventional cryostat technique and the combination of freezing and microwave-stimulated fixation. Acta Pathol Microbiol Immunol Scand. 1990; 98: 200–202.Google Scholar