Separation in Solution

  • Robert K. Scopes
Chapter
Part of the Springer Advanced Texts in Chemistry book series (SATC)

Abstract

The methods described in Chapters 4–7 involve phase changes. The proteins pass from liquid phase (dissolved) to solid (precipitated or adsorbed) and back again. Not all proteins withstand the stresses occurring in these methods; gentler methods in which the proteins remain in solution at all times are available. One of these, gel filtration, is one of the principal techniques used in purifying enzymes and other proteins, and it will be considered in detail. Other methods, grouped together as electrophoretic techniques, are less widely used, for reasons that will be outlined in Section 8.2. A third method of separation in solution involves phase partitioning where proteins may move from one liquid phase into another (Section 8.3), and a fourth method, ultrafiltration, is described in Section 8.4. As a general technique, separation in solution is an important procedure both in research and industrial protein purification.

Keywords

Migration Sucrose Starch Agar Agarose 

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Robert K. Scopes
    • 1
  1. 1.Department of Biochemistry and the Centre for Protein and Enzyme TechnologyLa Trobe UniversityBundooraAustralia

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