Does Intracellular pH Affect Vascular Tone?

  • C. Aalkjaer
Part of the Experimental Biology and Medicine book series (EBAM, volume 26)


It is well established that a reduction of extracellular pH (pHo) is associated with a reduction of tone in most vascular beds. It has though been difficult to assess the importance of intracellular pH (pHi) for this response mainly because it has previously been difficult to measure pHi in vascular smooth muscle and endothelial cells of the vasculature. Over recent years the availability of pH sensitive intracellularly trapped fluorescent dyes have, however, made it possible to address the role of pHi for vascular function and it is the purpose of this short paper to review the literature, which have addressed this question.


Vascular Tone Force Development Hypercapnic Acidosis Steady State Reduction Subcutaneous Small Artery 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    AALKJÆR, C. AND E. J. CRAGOE. Intracellular pH regulation in resting and contracting segments of rat mesenteric resistance vessels. J. Physiol. 402: 391–410, 1988.PubMedGoogle Scholar
  2. 2.
    AALKJÆR, C. AND A. HUGHES. Chloride and bicarbonate transport in rat resistance arteries. J. Physiol. 436: 57–73, 1991.PubMedGoogle Scholar
  3. 3.
    AALKJÆR, C. AND J. LOMBARD. Is pH, important for the response of rat small arteries to hypoxia? [abstract] J. Vasc. Res. 31 (Suppl 1): 1, 1994.CrossRefGoogle Scholar
  4. 4.
    AALKJÆR, C. AND M. J. MULVANY. Effect of changes in intracellular pH on the contractility of rat resistance vessels. Progress in Biochemical Pharmacology 23: 150–158, 1988.PubMedGoogle Scholar
  5. 5.
    AALKJÆR, C. AND M. J. MULVANY. Steady-state effects of arginine vasopressin on force and intracellular pH of isolated mesenteric resistance arteries from rats. Am. J. Physiol. 261: C1010 - C1017, 1991.PubMedGoogle Scholar
  6. 6.
    AICKIN, C. C. Direct measurement of intracellular pH and buffering power in smooth muscle cells of guinea-pig vas deferens. J. Physiol. 349: 571–585, 1984.PubMedGoogle Scholar
  7. 7.
    AICKIN, C. C. Mechanisms involved in control of intracellular pH in smooth muscle. Verhandlungen. der. Deutschen. Zoologischen. Gesellschaft. 82: 121–129, 1989.Google Scholar
  8. 8.
    AUSTIN, C. AND S. WRAY. Extracellular pH signals affect rat vascular tone by rapid transduction into intracellular pH changes. J Physiol. Lond. 466: 1–8, 1993.PubMedGoogle Scholar
  9. 9.
    BAMOSA, A. O., A. D. IGHOROJE, N. C. SPURWAY, AND M. J. TAGGART. Vascular tone variations, initiated by ammonium “pulses”, in an isolated, perfused rat tail preparation. J. Physiol. 392: 23P, 1987.Google Scholar
  10. 10.
    BERK, B. C., M. S. ARONOW, T. A. BROCK, E. CRAGOE, M. A. GIMBRONE, AND R. W. ALEXANDER. Angiotensin II-stimulated Na+ill+ exchange in cultured vascular smooth muscle cells. J. Biol. Chem. 262: 5057–5064, 1987.PubMedGoogle Scholar
  11. 11.
    DAUGHERTY, R. M., J. B. SCOTT, J. M. DABNEY, AND F. J. HADDY. Local effects of 02 and CO2 on limb, renal, and coronary vascular resistances. Am. J. Physiol. 213: 1102–1110, 1967.PubMedGoogle Scholar
  12. 12.
    FLEISCH, A., I. SIBUL, AND V. PONOMAREV. Kohlensaure und sauerstoff mangel als auslosende reize. Arch. Ges. Physiol. 230: 814–834, 1932.CrossRefGoogle Scholar
  13. 13.
    HARDER, D. R. Effect of W and elevated P(CO2) on membrane electrical properties of rat cerebral arteries. Pflügers Arch. 394: 182–185, 1982.PubMedCrossRefGoogle Scholar
  14. 14.
    HARDER, D. R. AND J. A. MADDEN. Cellular mechanism of force development in cat middle cerebral artery by reduced pCO2. Pflügers. Arch. Eur. J. Physiol. 403: 402–404, 1985.CrossRefGoogle Scholar
  15. 15.
    HATORI, N., B. P. FINE, A. NAKAMURA, E. CRAGOE, AND A. AVIV. Angiotensin II effect on cytosolic pH in cultured rat vascular smooth muscle cells. J. Biol. Chem. 262: 5073–5078, 1987.PubMedGoogle Scholar
  16. 16.
    IGHOROJE, A. D. AND N. C. SPURWAY. Procedures to acidify cytoplasm raise the tone of isolated (rabbit ear) blood vessels [abstract]. J. Physiol. 357: 105P, 1984.Google Scholar
  17. 17.
    IGHOROJE, A. D. AND N. C. SPURWAY. How does vascular muscle in the isolated rabbit ear adapt its tone after alkaline or acid loads. J. Physiol. 367: 46P, 1985.Google Scholar
  18. 18.
    IZZARD, A. S. AND A. M. HEAGERTY. The measurement of internal pH in resistance arterioles: evidence that intracellular pH is more alkaline in SHR than WKY animals. J Hypertens. 7: 173–180, 1989.PubMedGoogle Scholar
  19. 19.
    JENSEN, P. E., A. HUGHES, H. C. M. BOONEN, AND C. AALKJÆR. Force, membrane potential andi during activation of rat mesenteric small arteries with norepinephrine, potassium, aluminum fluoride and phorbol ester–effects of changes in pH,. Circ. Res. 73: 314–324, 1993.PubMedCrossRefGoogle Scholar
  20. 20.
    KONTOS, H. A., D. W. RICHARDSON, AND J. L. PATTERSON. Effects of hypercapnia on human forearm blood vessels. Am. J. Physiol. 212: 1070–1080, 1967.PubMedGoogle Scholar
  21. 21.
    KORBMACHER, C., H. HELBIG, F. STAHL, AND M. WIEDERHOLT. Evidence for Na/H exchange and Cl/HCO3 exchange in A10 vascular smooth muscle cells. Pflügers Arch. 412: 29–36, 1988.PubMedGoogle Scholar
  22. 22.
    LIU, B., R. MARTINEZ-SAGUILAN, R. J. GILLIES, AND P. C. JOHNSON. The effects of extracellular pH (pH) on the membrane potential of vascular smooth muscle [abstract]. J. Vasc. Res. 31 (Suppl 1): 29, 1994.Google Scholar
  23. 23.
    LOUTZENHISER, R., Y. MATSUMOTO, W. OKAWA, AND M. EPSTEIN. W-induced vasodilation of rat aorta is mediated by alterations in intracellular calcium sequestration. Circ. Res. 67: 426–439, 1990.PubMedCrossRefGoogle Scholar
  24. 24.
    MATTHEWS, J. G., J. E. GRAVES, AND L. POSTON. Relationships between pHi and tension in isolated rat mesenteric resistance arteries. J Vasc. Res. 29: 330–340, 1992.PubMedCrossRefGoogle Scholar
  25. 25.
    NIELSEN, H., C. AALKJIER, AND M. J. MULVANY. Differential effects of changes in carbon dioxide tension on contractile responses to noradrenaline in rat mesenteric resistance arteries. Pflügers. Arch. Eur. J. Physiol. 419: 51–56, 1991.CrossRefGoogle Scholar
  26. 26.
    ROOS, A. AND W. F. BORON. Intracellular pH. Physiol. Rev. 61: 296–434, 1981.PubMedGoogle Scholar
  27. 27.
    SIEGEL, G., C. KAMPE, AND B. J. EBELING. pH-dependent myogenic control in cerebral vascular smooth muscle. Cerebral. Microcirculation. and Metabolism 213–226, 1981.Google Scholar
  28. 28.
    SIGURDSSON, S. B. Comparison of portal vein responsiveness in tris, HEPES or bicarbonate-phosphate buffered media. Acta Pharmacol. Toxicol. 53: 81–87, 1983.CrossRefGoogle Scholar
  29. 29.
    TIAN, R., N. A. LASSEN, M. J. MULVANY, F. ANDREASEN, AND C. AALKJIER. Is pH, or pH, important for cerebral tone during hypercapnic acidosis? [abstract] J. Vasc. Res. 31 (Suppl 1): 52, 1994.Google Scholar
  30. 30.
    VIGNE, P., J. P. BREITTMAYER, C. FRELIN, AND M. LAZDUNSKI. Dual control of the intracellular pH in aortic smooth muscle cells by a cAMP-sensitive HCO3-/C1- antiporter and a protein kinase C-sensitive antiporter. J. Biol. Chem. 263: 18023–18029, 1988.PubMedGoogle Scholar
  31. 31.
    WEST, G. A., D. C. LEPPLA, AND J. M. SIMARD. Effects of external pH on ionic currents in smooth muscle cells from the basilar artery of the guinea pig. Circ. Res. 71: 201–209, 1992.PubMedCrossRefGoogle Scholar
  32. 32.
    WRAY, S. The effects of metabolic inhibition on uterine metabolism and intracellular pH in the rat. J. Physiol. 423: 411–423, 1990.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • C. Aalkjaer
    • 1
  1. 1.Department of PharmacologyUniversity of AarhusAarhus CDenmark

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