Contractile Responses of Mesenteric Resistance Arteries to BAY K 8644 in Mineralocorticoid-Salt Hypertension

  • Cathy A. Bruner
  • Carol Hollister
  • Carlos O. Rivera
Part of the Experimental Biology and Medicine book series (EBAM, volume 26)


Changes in the structure and function of blood vessels are hallmarks of hypertension. Until the recent advent of methods suitable for the study of small resistance arteries, most studies of isolated vascular function in hypertension were conducted on helically-cut strips or rings of large conduit arteries, such as aorta, carotid artery, tail artery, or femoral artery. The extent to which changes in vascular function demonstrated in such systems could be generalized to smaller, resistance arteries has been a matter of debate. The purpose of the experiments presented herein was to compare changes in in vitro vascular contractile sensitivity in conduit and resistance arteries from mineralocorticoid-salt hypertensive and normotensive rats. Contractile responsiveness of carotid arteries and mesenteric resistance arteries to norepinephrine and serotonin was determined. Furthermore, we determined contractile sensitivity to direct depolarization with potassium chloride and to activation of dihydropyridinesensitive voltage-operated calcium channels with BAY K 8644 [methyl-1,4-dihydro-2,6-dimethyl-3-nitro(2-trifluoromethylphenyl)pyridine-5-carboxylate]. We hypothesized that if a generalized change in vascular function is present in mineralocorticoid-salt hypertension, then alterations in contractile sensitivity similar to those seen in conduit arteries would be observed in resistance arteries.


Carotid Artery Potassium Chloride Contractile Response Conduit Artery Small Mesenteric Artery 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Asano, M., Aoki, K., and T. Matsuda. Contractile effects of Bay K 8644, a dihydropyridine calcium agonist, on isolated femoral arteries from spontaneously hypertensive rats. J. Pharm. Exp. Ther. 239: 198–205, 1986.Google Scholar
  2. 2.
    Bohr, D.F., Dominiczak, A.F., and R.C. Webb. Pathophysiology of the vasculature in hypertension. Hypertension 18 (Suppl. III): III69 - III74, 1991.Google Scholar
  3. 3.
    Borders, J.L. and H.J. Granger. Power dissipation as a measure of peripheral resistance in vascular networks. Hypertension 8: 184–191, 1986.PubMedCrossRefGoogle Scholar
  4. 4.
    Bruner, C.A. Vascular responsiveness in rats resistant to aldosterone-salt hypertension. Hypertension 20: 59–66, 1992.PubMedCrossRefGoogle Scholar
  5. 5.
    Bruner, C.A., Mangiapane, M.L., Fink, G.D., and R.C. Webb. Area postrema ablation and vascular reactivity in deoxycorticosterone-salt-treated rats. Hypertension 11: 668–673, 1988.PubMedCrossRefGoogle Scholar
  6. 6.
    Bruner, C.A. and R.C. Webb. Increased vascular reactivity to Bay K 8644 in genetic hypertension. Pharmacology 41: 24–35, 1990.PubMedCrossRefGoogle Scholar
  7. 7.
    Cheung, D.W. Membrane potential of vascular smooth muscle and hypertension in spontaneously hypertensive rats. Can. J. Physiol. Pharmacol. 62: 957–960, 1984.PubMedCrossRefGoogle Scholar
  8. 8.
    Harper, S.L. and H.G. Bohlen. Microvascular adaptation in the cerebral cortex of adult spontaneously hypertensive rats. Hypertension 6: 408–419, 1984.PubMedCrossRefGoogle Scholar
  9. 9.
    Hermsmeyer, K., Abel, P.W., and A.J. Trapani. Norepinephrine sensitivity and membrane potentials of caudal arterial muscle in DOCA-salt, Dahl, and SHR hypertension in the rat. Hypertension 4 (Suppl. II): II49 – II52, 1982.PubMedGoogle Scholar
  10. 10.
    Imig, J.D. and G.L. Anderson. Small artery resistance increases during the development of renal hypertension. Hypertension 17: 317–322, 1991.PubMedCrossRefGoogle Scholar
  11. 11.
    Katovich, M.J., Soltis, E.E., Iloege, E., and F.P. Field. Time course alterations in vascular adrenergic responsiveness in the DOCA-NaCl-treated rat. Pharmacology 29: 173–180, 1980.CrossRefGoogle Scholar
  12. 12.
    King, C.M. and R.C. Webb. The endothelium partially obscures enhanced microvessel reactivity in DOCA hypertensive rats. Hypertension 12: 420–427, 1988.PubMedCrossRefGoogle Scholar
  13. 13.
    Lamb, F.S. and R.C. Webb. Regenerative electrical activity and arterial contraction in hypertensive rats. Hypertension 13: 70–76, 1989.PubMedCrossRefGoogle Scholar
  14. 14.
    Longhurst, P.A., Rice, P.J., Taylor, D.A., and W.W. Fleming. Sensitivity of caudal arteries and the mesenteric vascular bed to norepinephrine in DOCA-salt hypertension. Hypertension 12: 133–142, 1988.PubMedCrossRefGoogle Scholar
  15. 15.
    McMahon, E. Garwitz and R.J. Paul. Calcium sensitivity of isometric force in intact and chemically skinned aortas during the development of aldosterone-salt hypertension in the rat. Circ. Res. 56: 427–435, 1985.PubMedCrossRefGoogle Scholar
  16. 16.
    Mecca, T.E., Lamb, F.S., Hall, J.L., and R.C. Webb. Cerebral intraventricular 6-hydroxydopamine prevents vascular changes in the mineralocorticoid hypertensive rat. Proc. Soc. Exp. Biol. Med. 179: 248–253, 1985.PubMedGoogle Scholar
  17. 17.
    Meininger, G.A., Harris, P.D., and I.G. Joshua. Distributions of microvascular pressure in skeletal muscle of one-kidney, one clip, two-kidney, one clip, and deoxycorticosterone-salt hypertensive rats. Hypertension 6: 27–34, 1984.PubMedCrossRefGoogle Scholar
  18. 18.
    Moreland, R.S., Lamb, F.S., Webb, R.C., and D. F. Bohr. Functional evidence for increased sodium permeability in aortas from DOCA hypertensive rats. Hypertension 6 (Suppl. I), I88 – I94, 1984.CrossRefGoogle Scholar
  19. 19.
    Moreland, R.S., Webb, R.C., and D.F. Bohr. Vascular changes in DOCA hypertension-influence of a low protein diet. Hypertension 4 (Suppl. III): III99 - III107, 1982.Google Scholar
  20. 20.
    Sada, T., Koike, H., Ikeda, M., Sato, K., Ozaki, H, and Karaki, H. Cytosolic free calcium of aorta in hypertensive rats. Chronic inhibition of angiotensin converting enzyme. Hypertension 16: 245–251, 1990.Google Scholar
  21. 21.
    Sada, T., Koike, H., and M. Miyamoto. Long-term inhibition of angiotensin converting enzyme suppresses calcium channel agonist-induced contraction of aorta in hypertensive rats. Hypertension 14: 652–659, 1989.PubMedCrossRefGoogle Scholar
  22. 22.
    Shoemaker, R.L. and H.W. Overbeck. Vascular smooth muscle membrane potential in rats with early and chronic one-kidney, one-clip hypertension. Proc. Soc. Exp. Biol. Med. 181: 529–534, 1986.PubMedGoogle Scholar
  23. 23.
    Smith, J.M., Jones, S.B., Bylund, D.B., and A.W. Jones. Characterization of the Alpha-1 adrenergic receptors in the thoracic aorta of control and aldosterone hypertensive rats: Correlation of radioligand binding with potassium efflux and contraction. J. Pharmacol. Exp. Ther. 241: 882–890, 1987.PubMedGoogle Scholar
  24. 24.
    Soltis, E.E., Newman, P.S., and J.W. Olson. Polyamines, vascular smooth muscle, and deoxycorticosterone acetate-salt hypertension. Hypertension 18: 85–92, 1991.PubMedCrossRefGoogle Scholar
  25. 25.
    Steele, T.H. and L. Challoner-Hue. Increased vascular response to calcium channel agonist by Dahl S rat kidney. Am. J. Physiol. 254: 652–659, 1989.Google Scholar
  26. 26.
    Storm, D.S., Stuenkel, E.L., and R.C. Webb. Calcium channel activation in arterioles from genetically hypertensive rats. Hypertension 20: 380–388, 1992.PubMedCrossRefGoogle Scholar
  27. 27.
    Storm, D.S., Turla, M.B., Todd, K.M., and R.C. Webb. Contractile responses to the calcium channel agonist, Bay K 8644, in aortae from rats with coarctation-induced hypertension. Am. J. Hypertens. 3: 245S - 248S, 1989.Google Scholar
  28. 28.
    Storm, D.S., and R.C. Webb. Alpha-adrenergic receptors and 45Ca2+ efflux in arteries from deoxycorticosterone acetate hypertensive rats. Hypertension 19: 734–738, 1992.PubMedCrossRefGoogle Scholar
  29. 29.
    Storm, D.S. and R.C. Webb. Contractile responses to Bay K 8644 in rats with coarctation-induced hypertension. Proc. Soc. Exp. Biol. Med. 203: 92–99, 1993.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Cathy A. Bruner
    • 1
  • Carol Hollister
    • 1
  • Carlos O. Rivera
    • 1
  1. 1.Dept. of PharmacologyAlbany Medical CollegeAlbanyUSA

Personalised recommendations