Confocal Myography: Applications for the Study of Resistance Arteries
The development of confocal microscopes has enabled the collection and reconstruction of serial ‘optical’ sections of labelled unfixed specimens (1). Using fluorescent nuclear dyes and conventional fluorescence microscopy it is possible to identify cell type, location and viability within living myograph-mounted resistance arteries (2). We have now extended this technique using confocal microscopy and a variety of intracellular and extracellular stains. Many in-vitro experiments on resistance arteries rely on the integrated response of all of the cells in an isolated segment. This response may be either a change in diameter, isometric force or total fluorescence of a given indicator. It has been our aim to develop techniques which will allow visualisation of individual cells within a myograph mounted vessel in order to assess each cell’s activity. We have now identified a number of possible methods which may help to answer some of the questions which cannot be addressed using currently available techniques.
KeywordsResistance Artery Frame Average Physiological Salt Solution Conventional Fluorescence Microscopy Mesenteric Resistance Artery
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