Xenopus Oocytes as an Expression System for Ion Channels
Xenopus oocytes have become one of the most commonly used systems in which to express and study ion channels and neural receptors. Gurdon et al. (1971) first demonstrated that Xenopus oocytes can be used to express exogenous mRNA species by injecting reticulocyte RNA into the cytoplasm and studying the synthesis of hemoglobin using biochemical techniques. Miledi and co-workers then demonstrated that functional ion channels and receptors could be expressed by injecting rat brain RNA and using a voltage clamp to measure sodium channel and nicotinic acetylcholine receptor (nAChR) activity (Barnard et al., 1982; Miledi et al., 1982; Gundersen et al., 1984). Many investigators have since used Xenopus oocytes to study the expression and translation of a large number of molecules [for reviews see Gurdon and Wickens (1983), Colman (1984), Soreq (1985), Snutch (1988), and Dascal (1987)].
KeywordsSodium Channel Xenopus Oocyte Follicle Cell Tissue Culture Dish Guanidine Hydrochloride
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