The Prokaryotes pp 3948-3951 | Cite as

The Genus Propionigenium

  • Bernhard Schink


The genus Propionigenium consists so far of one single species that comprises four strains of physiologically and morphologically similar isolates from various origins (Schink and Pfennig, 1982). This genus was created to house strictly anaerobic bacteria that are able to grow by decarboxylation of succinate to propionate. Enrichment cultures, which were set up originally to enrich for syntrophic succinate degraders from marine and freshwater sediments, developed unexpectedly fast growth of small, coccoid bacteria that did not depend on cooperation with hydrogen-scavenging partners and formed propionate as the sole fermentation product. Pure cultures could only be obtained with enrichment cultures from marine sources; the freshwater enrichments grew much slower, and pure cultures were finally isolated when the sodium chloride concentration of the medium was enhanced to 100–150 mM. This finding gave the first hint on a sodium dependence of this new type of energy conservation.


Enrichment Culture Freshwater Sediment Sodium Chloride Concentration Decarboxylation Reaction Coccoid Bacterium 


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Literature Cited

  1. Anantharam, V., M. J. Allison, and P. C. Maloney. 1989. Oxalate: formate exchange. The basis for energy coupling in Oxalobacter. J. Biol. Chem. 264: 7244–7250.PubMedGoogle Scholar
  2. Balch, W. E., G. E. Fox, L. J. Magrum, C. R. Woese, and R. S. Wolfe. 1979. Methanogens: reevaluation of a unique biological group. Microbiol. Rev. 43: 260–296.PubMedPubMedCentralGoogle Scholar
  3. Brown, I. I., M. J. Galperin, A. N. Glagolev, and V. P. Skulachev. 1983. Utilization of energy stored in the form of Na- and K+ ion gradients by bacterial cells. Eur. J. Biochem. 134: 345–349.PubMedCrossRefGoogle Scholar
  4. Delwiche, E. A., J. J. Pestka, and M. L. Tortorello. 1985. The Veillonellae: Gram-negative cocci with a unique physiology. Ann. Rev. Microbiol. 39: 175–193.CrossRefGoogle Scholar
  5. Denger, K., and B. Schink. 1990. New motile anaerobic bacteria growing by succinate decarboxylation to propionate. Arch. Microbiol. (in press)Google Scholar
  6. Dibrov, P. A., V. A. Kostyrko, R. L. Lazarova, V. P. Skulachev, and I. A. Smirnova. 1986b. The sodium cycle. I. Na’-dependent motility and modes of membrane energetization in the marine alkalotolerant Vibrio alginolyticus. Biochim. Biophys. Acta 850: 449–457.PubMedCrossRefGoogle Scholar
  7. Dibrov, P. A., R. L. Lazarova, V. P. Skulachev, and M. L. Verkhovskaya. 1986a. The sodium cycle. II. Na+-coupled oxidative phosphorylation in Vibrio alginolyticus cells. Biochim. Biophys. Acta 850: 458–465.Google Scholar
  8. Dimroth, P., and W. Hilpert. 1984. Carboxylation of pyruvate and acetyl coenzyme A by reversal of the Na+ pumps oxaloacetate decarboxylase and methylmalonylCoA decarboxylase. Biochemistry 23: 5360–5371.CrossRefGoogle Scholar
  9. Hilpert, W., and P. Dimroth. 1982. Conversion of the chemical energy of methylmalonyl-CoA decarboxylation into a Na+ gradient. Nature 296: 584–585.PubMedCrossRefGoogle Scholar
  10. Hilpert, W., B. Schink, and P. Dimroth. 1984. Life by a new energy conservation mechanism with Na+ as coupling ion. EMBO-J. 3: 1665–1670.PubMedPubMedCentralGoogle Scholar
  11. Laubinger, W., and P. Dimroth. 1987. Characterization of the Na+-stimulated ATPase of Propionigenium modes-turn as an enzyme of the F, Fx type. Eur. J. Biochem. 168: 475–480.CrossRefGoogle Scholar
  12. Laubinger, W., and R Dimroth. 1988. Characterization of the ATP synthase of Propionigenium modestum as a primary sodium pump. Biochemistry 27: 7531–7537.PubMedCrossRefGoogle Scholar
  13. Mays, T. D., L. V. Holdeman, W. E. C. Moore, M. Rogosa, and J. L. Johnson. 1982. Taxonomy of the genus Veillonella Prévot. Int. J. Syst. Bacteriol. 32: 28–36.CrossRefGoogle Scholar
  14. Pfennig, N. 1978. Rhodocyclus puxpureus gen. nov. and sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int. J. Syst. Bacteriol. 28: 283–288.CrossRefGoogle Scholar
  15. Scheifinger, C. C., and M. J. Wolin. 1973. Propionate formation from cellulose and soluble sugars by combined cultures of Bacteroides succinogenes and Selenomonas ruminantium. Appl. Microbiol. 26: 789–795.PubMedPubMedCentralGoogle Scholar
  16. Schink, B., and N. Pfennig. 1982. Propionigenium modes-turn gen. nov. sp. nov., a new strictly anaerobic, nonsporing bacterium growing on succinate. Arch. Microbiol. 133: 209–216.Google Scholar
  17. Skulachev, V. P. 1985. Membrane-linked energy transductions. Bioenergetic functions of sodium: H+ is not unique as a coupling ion. Eur. J. Biochem. 151: 199–208.PubMedCrossRefGoogle Scholar
  18. Thauer, R. K., K. Jungermann, K. Decker. 1977. Energy conservation in chemotrophic anaerobic bacteria. Bacteriol. Rev. 41: 100–180.PubMedPubMedCentralGoogle Scholar
  19. Thauer, R. K., J. G. Morris. 1984. Metabolism of chemotrophic anaerobes: old views and new aspects, p. 123–168. In: D. P. Kelly, and N. G. Carr, (ed.), The microbe 1984. Part II. Prokaryotes and eukaryotes, Soc. Gen. Microbiol. Symp. vol. 36. Cambridge University Press, Cambridge, UK.Google Scholar
  20. Widdel, E, G. W. Kohring, and F. Mayer. 1983. Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. III. Characterization of the filamentous gliding Desulfonema limicola gen. nov. sp. nov. and Desulfonema magnum sp. nov. Arch. Microbiol. 134: 286–294.CrossRefGoogle Scholar
  21. Widdel, F., and N. Pfennig. 1981. Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. Isolation of new sulfate-reducing bacteria enriched with acetate from saline environments. Description of Desulfobacter postgatei gen. nov. sp. nov. Arch. Microbiol. 129: 395–400.PubMedCrossRefGoogle Scholar
  22. Wolin, M. J. 1979. The rumen fermentation: a model for microbial interactions in anaerobic systems. Adv. Microb. Ecol. 3: 49–77.CrossRefGoogle Scholar
  23. Yousten, A. A., and E. A. Delwiche. 1961. Biotin and vitamin B12 coenzymes in succinate decarboxylation by Propionibacterium pentosaceum and Veillonella alcalescens. Bacteriol. Proc. 61: 175.Google Scholar

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© Springer Science+Business Media New York 1992

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  • Bernhard Schink

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