Analysis of Three-Dimensional Image Data: Display and Feature Tracking
Microscopes have been used for almost two centuries to produce a wealth of biological information based on direct observation and photography. Recent advancements in digital image processing and display have created a revolution in biological and medical imaging. Digital methods have lead to significant improvements in the ease of image acquisition and data quality for both light and electron microscopy. It is now possible to routinely obtain quantitative image data and not simply pictures. The advent of quantitative microscopy is a major step forward in the application, and utilization of structural information for biological problems at the level of cells and cell components. Perhaps most significant has been the possibility of examining complex, noncrystalline objects such as supra molecular assemblies in three dimensions. Combined with powerful new probes to examine specific molecular components, three-dimensional imaging can provide new insights into the spatial localization patterns of specific molecules and their redistribution, for example during the cell cycle or development.
KeywordsMatched Filter Volumetric Data Stereo Pair Drosophila Embryo Chromatin Fiber
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