Introduction: Principles of Electron Tomography

  • Joachim Frank


Tomography is a method for reconstructing the interior of an object from its projections. The word tomography literally means the visualization of slices, and is applicable, in the strict sense of the word, only in the narrow context of a single-axis tilt geometry: e.g., in medical computerized axial tomography (CAT-scan imaging), the detector-source arrangement is tilted relative to the patient around a single axis. In electron microscopy, where the beam direction is fixed, the specimen holder is tilted around a single axis (Fig. 1). However, the usage of this term has recently become more liberal, encompassing arbitrary geometries. In line with this relaxed convention, we will use the term electron tomography for any technique that employs the transmission electron microscope to collect projections of an object and uses these projections to reconstruct the object in its entirety.


Fourier Space Single Axis Word Tomography Macromolecular Assembly Angular Increment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Joachim Frank
    • 1
    • 2
  1. 1.Wadsworth Center for Laboratories and ResearchNew York State Department of HealthAlbanyUSA
  2. 2.Department of Biomedical Sciences, School of Public HealthState University of New York at AlbanyAlbanyUSA

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