Intracellular Labeling and Immunocytochemistry

  • Stephen T. Kitai
  • G. Richard Penny
  • Howard T. Chang


Since its introduction in 1976 (Cullheim and Kellerth, 1976; Jankowska et al., 1976; Kitai et al., 1976; Light and Durkovic, 1976; Snow et al., 1976), the method of intracellular injection of horseradish peroxidase (HRP) has established itself as an enormously productive tool for neurobiology (Kitai and Bishop, 1981; Kitai and Wilson, 1982). The fundamental advantage of the technique is that it allows direct correspondence between cellular physiology and morphology to be established. First, as a physiological tool, the HRP-filled microelectrode is suitable for the analysis of any neurophysiological property of a neuron that can be assayed by intracellular recording. Second, as a morphological tool, intracellular iontophoresis of HRP fills and labels the entire extent of a neuron, including soma, dendrites, dendritic specializations such as spines, and as much of the axon, axonal collaterals, and terminals as survival time permits. The morphological rendition of the HRP-filled neuron revealed by enzyme histochemistry is equal to or better than the results of the very best Golgi stains.


Tyrosine Hydroxylase Primary Antiserum Intracellular Injection Tissue Chopper Intracellular Application 


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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Stephen T. Kitai
    • 1
  • G. Richard Penny
    • 1
  • Howard T. Chang
  1. 1.Department of Anatomy and Neurobiology, College of MedicineUniversity of TennesseeTennesseeUSA

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